ArticleBiophysical journal2009
Fretting about FRET: failure of the ideal dipole approximation.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Characterization of Ligand Binding in Human Serum Albumin from Atomistic Energy Transfer Simulations.Small methods · 2025Article
- Pursuing excitonic energy transfer with programmable DNA-based optical breadboards.Chemical Society reviews · 2023Review
- Photoswitchable Molecular Units with Tunable Nonlinear Optical Activity: A Theoretical Investigation.Molecules (Basel, Switzerland) · 2023Article
- Self-Quenching Behavior of a Fluorescent Probe Incorporated within Lipid Membranes Explored Using Electrophoresis and Fluorescence Lifetime Imaging Microscopy.The journal of physical chemistry. B · 2023Article
- Tunable Electronic Structure via DNA-Templated Heteroaggregates of Two Distinct Cyanine Dyes.The journal of physical chemistry. C, Nanomaterials and interfaces · 2022Article
- Determination of FRET orientation factor between artificial fluorophore and photosynthetic light-harvesting 2 complex (LH2).Scientific reports · 2022Article
- Accurate Modeling of Excitonic Coupling in Cyanine Dye Cy3.The journal of physical chemistry. A · 2021Article
- Fluorogenic aptamers resolve the flexibility of RNA junctions using orientation-dependent FRET.RNA (New York, N.Y.) · 2021Article
- Enhancement of Energy Transfer Efficiency with Structural Control of Multichromophore Light-Harvesting Assembly.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Loop-closure kinetics reveal a stable, right-handed DNA intermediate in Cre recombination.Nucleic acids research · 2020Article
- In Vivo Biosensing Using Resonance Energy Transfer.Biosensors · 2019Review
- Disentanglement of excited-state dynamics with implications for FRET measurements: two-dimensional electronic spectroscopy of a BODIPY-functionalized cavitand.Chemical science · 2018Article
- Theory of FRET "Spectroscopic Ruler" for Short Distances: Application to Polyproline.The journal of physical chemistry. B · 2018Article
- Challenging FRET-based E-Cadherin force measurements in Drosophila.Scientific reports · 2017Article
- Oxidative species-induced excitonic transport in tubulin aromatic networks: Potential implications for neurodegenerative disease.Journal of photochemistry and photobiology. B, Biology · 2017Article
- Orientation-dependent FRET system reveals differences in structures and flexibilities of nicked and gapped DNA duplexes.Nucleic acids research · 2017Article
- Förster resonance energy transfer: Role of diffusion of fluorophore orientation and separation in observed shifts of FRET efficiency.PloS one · 2017Article
- Predicting signatures of anisotropic resonance energy transfer in dye-functionalized nanoparticles.RSC advances · 2016Article
- Revealing Nucleic Acid Mutations Using Förster Resonance Energy Transfer-Based Probes.Sensors (Basel, Switzerland) · 2016Review
- Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
With recent growth in the use of fluorescence-detected resonance energy transfer (FRET), it is being applied to complex systems in modern and diverse ways where it is not always clear that the common approximations required for analysis are applicable. For instance, the ideal dipole approximation (IDA), which is implicit in the Förster equation, is known to break down when molecules get "too close" to each other. Yet, no clear definition exists of what is meant by "too close". Here we examine several common fluorescent probe molecules to determine boundaries for use of the IDA. We compare the Coulombic coupling determined essentially exactly with a linear response approach with the IDA coupling to find the distance regimes over which the IDA begins to fail. We find that the IDA performs well down to roughly 20 A separation, provided the molecules sample an isotropic set of relative orientations. However, if molecular motions are restricted, the IDA performs poorly at separations beyond 50 A. Thus, isotropic probe motions help mask poor performance of the IDA through cancellation of error. Therefore, if fluorescent probe motions are restricted, FRET practitioners should be concerned with not only the well-known kappa2 approximation, but also possible failure of the IDA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.