ArticleBiochemistry2009
FtsZ filament dynamics at steady state: subunit exchange with and without nucleotide hydrolysis.
Article in Biochemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 74 citations in OpenAlex.
- Structure insight into FtsZ function maintaining under acid stress of Streptococcus mutans.International journal of oral science · 2026Article
- Bacteriophage P1 protein Icd inhibits bacterial division by targeting FtsZ.Frontiers in microbiology · 2025Article
- PA5402-5407 of Pseudomonas aeruginosa Regulate the Expression of the Aa3-Type Oxidases and Their Growth Under Carbon Starvation and High-Density Conditions.Current microbiology · 2024Article
- How Does the Spatial Confinement of FtsZ to a Membrane Surface Affect Its Polymerization Properties and Function?Frontiers in microbiology · 2022Review
- How Do MinC-D Copolymers Act on Z-Ring Localization Regulation? A New Model ofFrontiers in microbiology · 2022Article
- Simulations of Proposed Mechanisms of FtsZ-Driven Cell Constriction.Journal of bacteriology · 2021Article
- A Novel Z-Ring Associated Protein ZapA-Like Protein (PA5407) FromFrontiers in microbiology · 2021Article
- The Assembly Switch Mechanism of FtsZ Filament Revealed by All-Atom Molecular Dynamics Simulations and Coarse-Grained Models.Frontiers in microbiology · 2021Article
- Assembly properties of bacterial tubulin homolog FtsZ regulated by the positive regulator protein ZipA and ZapA from Pseudomonas aeruginosa.Scientific reports · 2020Article
- A Unified Model for Treadmilling and Nucleation of Single-Stranded FtsZ Protofilaments.Biophysical journal · 2020Article
- A key bacterial cytoskeletal cell division protein FtsZ as a novel therapeutic antibacterial drug target.Bosnian journal of basic medical sciences · 2020Review
- Insights into the Structure, Function, and Dynamics of the Bacterial Cytokinetic FtsZ-Ring.Annual review of biophysics · 2020Review
- Assembly properties of the bacterial tubulin homolog FtsZ from the cyanobacteriumThe Journal of biological chemistry · 2019Article
- The E. coli MinCDE system in the regulation of protein patterns and gradients.Cellular and molecular life sciences : CMLS · 2019Review
- MinC N- and C-Domain Interactions Modulate FtsZ Assembly, Division Site Selection, and MinD-Dependent Oscillation inJournal of bacteriology · 2019Article
- FtsZ filaments have the opposite kinetic polarity of microtubules.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- The chloroplast division protein ARC6 acts to inhibit disassembly of GDP-bound FtsZ2.The Journal of biological chemistry · 2018Article
- Efficient models of polymerization applied to FtsZ ring assembly inProceedings of the National Academy of Sciences of the United States of America · 2018Article
- The Molecular Machinery of Chloroplast Division.Plant physiology · 2018Review
- Conserved Dynamics of Chloroplast Cytoskeletal FtsZ Proteins Across Photosynthetic Lineages.Plant physiology · 2018Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We have measured three aspects of FtsZ filament dynamics at steady state: rates of GTP hydrolysis, subunit exchange between protofilaments, and disassembly induced by dilution or excess GDP. All three reactions were slowed with an increase in the potassium concentration from 100 to 500 mM, via replacement of potassium with rubidium, or with an increase in the magnesium concentration from 5 to 20 mM. Electron microscopy showed that the polymers assembled under the conditions of fastest assembly were predominantly short, one-stranded protofilaments, whereas under conditions of slower dynamics, the protofilaments tended to associate into long, thin bundles. We suggest that exchange of subunits between protofilaments at steady state involves two separate mechanisms: (1) fragmentation or dissociation of subunits from protofilament ends following GTP hydrolysis and (2) reversible association and dissociation of subunits from protofilament ends independent of hydrolysis. Exchange of nucleotides on these recycling subunits could give the appearance of exchange directly into the polymer. Several of our observations suggest that exchange of nucleotide can take place on these recycling subunits, but not directly into the FtsZ polymer. Annealing of protofilaments was demonstrated for the L68W mutant in EDTA buffer but not in Mg buffer, where rapid cycling of subunits may obscure the effect of annealing. We also reinvestigated the nucleotide composition of FtsZ polymers at steady state. We found that the GDP:GTP ratio was 50:50 for concentrations of GTP >100 microM, significantly higher than the 20:80 ratio previously reported at 20 microM GTP.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.