ArticleNature structural & molecular biology2009
Localized thermodynamic coupling between hydrogen bonding and microenvironment polarity substantially stabilizes proteins.
Article in Nature structural & molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed.
- Backbone double-mutant cycle analysis quantifies hydrogen-bond energies in proteins.Protein science : a publication of the Protein Society · 2026Article
- InteracTor: Feature engineering and explainable AI for profiling protein structure-interaction-function relationships.PLoS computational biology · 2025Article
- Developing a Machine Learning Model for Hydrogen Bond Acceptance Based on Natural Bond Orbital Descriptors.The Journal of organic chemistry · 2025Article
- Structural Basis for Long Residence Time c-Src Antagonist: Insights from Molecular Dynamics Simulations.International journal of molecular sciences · 2024Article
- Inhibitor design for TMPRSS2: insights from computational analysis of its backbone hydrogen bonds using a simple descriptor.European biophysics journal : EBJ · 2024Article
- Quantum biological insights into CRISPR-Cas9 sgRNA efficiency from explainable-AI driven feature engineering.Nucleic acids research · 2023Article
- The Role of H-Bonds in the Excited-State Properties of Multichromophoric Systems: Static and Dynamic Aspects.Molecules (Basel, Switzerland) · 2023Review
- Intermolecular Interactions in G Protein-Coupled Receptor Allosteric Sites at the Membrane Interface from Molecular Dynamics Simulations and Quantum Chemical Calculations.Journal of chemical information and modeling · 2022Article
- Structure-activity relationships of mitochondria-targeted tetrapeptide pharmacological compounds.eLife · 2022Article
- Metabolomics analysis of milk thistle lipids to identify drought-tolerant genes.Scientific reports · 2022Article
- Switchable bifunctional molecular recognition in water using a pH-responsive Endo-functionalized cavity.Nature communications · 2022Article
- Prerequisites for Stabilizing Long-Range Synergistic Interactions amongBiochemistry · 2022Article
- Structural basis of DNA methylation-dependent site selectivity of the Epstein-Barr virus lytic switch protein ZEBRA/Zta/BZLF1.Nucleic acids research · 2022Article
- Temperature Dependence of Hydrophobic and Hydrophilic Forces and Interactions.The journal of physical chemistry. B · 2021Article
- PEGylation Increases the Strength of a Nearby NH-π Hydrogen Bond in the WW Domain.Biochemistry · 2021Article
- Targeted delivery of heat shock protein 90 inhibitors prevents growth of HER2-positive tumor.Biomaterials · 2021Article
- Protein nanofibril design via manipulation of hydrogen bonds.Communications chemistry · 2021Article
- Stereoelectronic effects in stabilizing protein-N-glycan interactions revealed by experiment and machine learning.Nature chemistry · 2021Article
- Article
- Polyol and sugar osmolytes can shorten protein hydrogen bonds to modulate function.Communications biology · 2020Article
12 more citing papers are in PubMed but not listed here.
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4 authors.
Funding
Abstract
The energetic contributions of hydrogen bonding to protein folding are still unclear, despite more than 70 years of study. This is due partly to the difficulty of extracting thermodynamic information about specific interactions from protein mutagenesis data and partly to the context dependence of hydrogen bond strengths. Herein, we test the hypothesis that hydrogen bond strengths depend on the polarity of their microenvironment, with stronger hydrogen bonds forming in nonpolar surroundings. Double-mutant cycle analysis using a combination of amide-to-ester backbone mutagenesis and traditional side chain mutagenesis revealed that hydrogen bonds can be stronger by up to 1.2 kcal mol(-1) when they are sequestered in hydrophobic surroundings than when they are solvent exposed. Such large coupling energies between hydrogen bond strengths and local polarity suggest that the context dependence of hydrogen bond strengths must be accounted for in any comprehensive account of the forces responsible for protein folding.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.