ArticleEukaryotic cell2009
Cooperative regulation of ADE3 transcription by Gcn4p and Bas1p in Saccharomyces cerevisiae.
Article in Eukaryotic cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 15 citations in OpenAlex.
- Selection markers for transformation of the sequenced reference monokaryon Okayama 7/#130 and homokaryon AmutBmut ofFungal biology and biotechnology · 2020Article
- Dissection of the PHO pathway in Schizosaccharomyces pombe using epistasis and the alternate repressor adenine.Current genetics · 2015Article
- Gene coexpression measures in large heterogeneous samples using count statistics.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- The identification of putative RNA polymerase II C-terminal domain associated proteins in red and green algae.Transcription · 2014Article
- Gcn4p-mediated transcriptional repression of ribosomal protein genes under amino-acid starvation.The EMBO journal · 2011Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The one-carbon response regulon is essential for the biosynthesis of nucleic acids as well as several amino acids. The ADE3 gene is known to encode a crucial one-carbon regulon enzyme, tetrahydrofolate synthase, which is involved in the biosynthesis of purine and the amino acids methionine and glycine. Therefore, the mechanism through which ADE3 transcription is regulated appears to be critical for the cross-talk among these metabolic pathways. Even so, the direct involvement of ADE3 transcription through gene-specific transcription factors has not been shown clearly. In this study, the promoter structure of the ADE3 gene was investigated in detail, and a genuine Gcn4p responsive element (GCRE) was confirmed among three putative GCRE elements in vivo and in vitro. Through gene deletion studies of Gcn4p and Bas1p, it was established that both factors are involved in the transcriptional regulation of the ADE3 gene. Direct binding to this GCRE and the occupancy of the ADE3 promoter by these factors were also confirmed. Taking these results together, we concluded that Gcn4p is responsible for the basal and inducible expression of the ADE3 gene, while Bas1p is required for its basal expression.
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