ArticleAmerican journal of physiology. Heart and circulatory physiology2009
Inhibition of myosin light chain phosphorylation decreases rat mesenteric lymphatic contractile activity.
Article in American journal of physiology. Heart and circulatory physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 65 citations in OpenAlex.
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- Edema and lymphatic clearance: molecular mechanisms and ongoing challenges.Clinical science (London, England : 1979) · 2023Review
- Intestinal Lymphatic Dysfunction in Kidney Disease.Circulation research · 2023Review
- Engineering approaches to investigate the roles of lymphatics vessels in rheumatoid arthritis.Microcirculation (New York, N.Y. : 1994) · 2023Review
- Lymphatic Clearance and Pump Function.Cold Spring Harbor perspectives in medicine · 2023Review
- KAmerican journal of physiology. Cell physiology · 2022Review
- Effect of Human Synovial Fluid From Osteoarthritis Patients and Healthy Individuals on Lymphatic Contractile Activity.Journal of biomechanical engineering · 2022Article
- A vascular smooth muscle-specific integrin-α8 Cre mouse for lymphatic contraction studies that allows male-female comparisons and avoids visceral myopathy.Frontiers in physiology · 2022Article
- Lymphatic Collecting Vessel: New Perspectives on Mechanisms of Contractile Regulation and Potential Lymphatic Contractile Pathways to Target in Obesity and Metabolic Diseases.Frontiers in pharmacology · 2022Review
- A multiscale sliding filament model of lymphatic muscle pumping.Biomechanics and modeling in mechanobiology · 2021Article
- Cartiotonic steroids affect monolayer permeability in lymphatic endothelial cells.Molecular and cellular biochemistry · 2021Article
- Dichotomous effects on lymphatic transport with loss of caveolae in mice.Acta physiologica (Oxford, England) · 2021Article
- Roles of sarcoplasmic reticulum CaScientific reports · 2020Article
- Evidence of functional ryanodine receptors in rat mesenteric collecting lymphatic vessels.American journal of physiology. Heart and circulatory physiology · 2019Article
- Ano1 mediates pressure-sensitive contraction frequency changes in mouse lymphatic collecting vessels.The Journal of general physiology · 2019Article
- Targeting lymphatic function as a novel therapeutic intervention for rheumatoid arthritis.Nature reviews. Rheumatology · 2018Review
- Experimental Models Used to Assess Lymphatic Contractile Function.Lymphatic research and biology · 2017Review
- Hyperglycemia- and hyperinsulinemia-induced insulin resistance causes alterations in cellular bioenergetics and activation of inflammatory signaling in lymphatic muscle.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2017Article
- Synchronization and Random Triggering of Lymphatic Vessel Contractions.PLoS computational biology · 2016Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Muscular lymphatics use both phasic and tonic contractions to transport lymph for conducting their vital functions. The molecular mechanisms regulating lymphatic muscle contractions are not well understood. Based on the well-established finding that the phosphorylation of myosin light chain 20 (MLC(20)) plays an essential role in blood vessel smooth muscle contraction, we investigated if phosphorylated MLC(20) (pMLC(20)) would modulate the tonic and/or phasic contractions of lymphatic muscle. The effects of ML-7, a MLC kinase inhibitor (1-10 microM), were tested on the contractile parameters of isolated and cannulated rat mesenteric lymphatics during their responses to the known modulators, pressure (1-5 cm H(2)O) and substance P (SP; 10(-7) M). Immunohistochemical and Western blot analyses of pMLC(20) were also performed on isolated lymphatics. The results showed that 1) increasing pressure decreased both the lymphatic tonic contraction strength and pMLC(20)-to-MLC(20) ratio; 2) SP increased both the tonic contraction strength and phosphorylation of MLC(20); 3) ML-7 decreased both the lymphatic tonic contraction strength and pMLC(20)-to-MLC(20) ratio; and 4) the increase in lymphatic phasic contraction frequency in response to increasing pressure was diminished by ML-7; however, the phasic contraction amplitude was not significantly altered by ML-7 either in the absence or presence of SP. These data provide the first evidence that tonic contraction strength and phasic contraction amplitude of the lymphatics can be differentially regulated, whereby the increase in MLC(20) phosphorylation produces an activation in the tonic contraction without significant changes in the phasic contraction amplitude. Thus, tonic contraction of rat mesenteric lymphatics appears to be MLC kinase dependent.
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