ArticlePlant physiology2009
A novel cation-dependent O-methyltransferase involved in anthocyanin methylation in grapevine.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
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Who cites it
79 citing papers in PubMed, 173 citations in OpenAlex.
- A Golgi-localized N-methyltransferase and reversible aldo-keto reductases coordinate dual terminal routes in galanthamine biosynthesis.The Plant journal : for cell and molecular biology · 2026Article
- Four-year field study reveals variable effects of phytohormone- and natural-based elicitors on anthocyanin metabolism in Tempranillo grapes.Journal of the science of food and agriculture · 2025Article
- Lonicera caerulea genome reveals molecular mechanisms of freezing tolerance and anthocyanin biosynthesis.Journal of advanced research · 2025Article
- Characterization of the glucosylated anthocyanin profile of 27 red grape (Frontiers in plant science · 2025Article
- Genome-Wide Characterization ofGenes · 2024Article
- Haplotype-resolved genome assembly and implementation of VitExpress, an open interactive transcriptomic platform for grapevine.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A transient expression tool box for anthocyanin biosynthesis in Nicotiana benthamiana.Plant biotechnology journal · 2024Article
- Unravelling and reconstructing the biosynthetic pathway of bergenin.Nature communications · 2024Article
- Genomic and transcriptomic studies on flavonoid biosynthesis in Lagerstroemia indica.BMC plant biology · 2024Article
- Genome-wide identification, expression profiling, and protein interaction analysis of the CCoAOMT gene family in the tea plant (Camellia sinensis).BMC genomics · 2024Article
- Harnessing the diversity of a lettuce wild relative to identify anthocyanin-related genes transcriptionally responsive to drought stress.Frontiers in plant science · 2024Article
- Metabolite and transcriptome analyses reveal the effects of salinity stress on the biosynthesis of proanthocyanidins and anthocyanins in grape suspension cells.Frontiers in plant science · 2024Article
- Model-assisted analysis for tuning anthocyanin composition in grape berries.Annals of botany · 2023Article
- Insight into the Molecular Mechanism of Flower Color Regulation inPlants (Basel, Switzerland) · 2023Article
- A multifunctional true caffeoyl coenzyme A O-methyltransferase enzyme participates in the biosynthesis of polymethoxylated flavones in citrus.Plant physiology · 2023Article
- Genome-wide identification of thePeerJ · 2023Article
- Evolution and diversification of the O-methyltransferase (OMT) gene family in Solanaceae.Genetics and molecular biology · 2023Article
- Functional Diversification and Structural Origins of Plant Natural Product Methyltransferases.Molecules (Basel, Switzerland) · 2022Review
- Maize Flavonoid Biosynthesis, Regulation, and Human Health Relevance: A Review.Molecules (Basel, Switzerland) · 2022Review
- Function deficiency of GhOMT1 causes anthocyanidins over-accumulation and diversifies fibre colours in cotton (Gossypium hirsutum).Plant biotechnology journal · 2022Article
19 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Anthocyanins are major pigments in colored grape (Vitis vinifera) berries, and most of them are monomethoxylated or dimethoxylated. We report here the functional characterization of an anthocyanin O-methyltransferase (AOMT) from grapevine. The expression pattern in two cultivars with different anthocyanin methylation profiles (Syrah and Nebbiolo) showed a peak at start ripening (véraison), when the concentrations of all methylated anthocyanins begin to increase. The purified recombinant AOMT protein was active on both anthocyanins and flavonols in vitro, with K(m) in the micromolar range, and was dependent on divalent cations for activity. AOMT showed a preference for 3',5' methylation when a 3',4',5' hydroxylated anthocyanin substrate was tested. In order to assess its in planta activity, we performed transient expression of AOMT in tobacco (Nicotiana benthamiana) leaves expressing the Production of Anthocyanin Pigment1 (PAP1) transcription factor from Arabidopsis (Arabidopsis thaliana). PAP1 expression in leaves induced the accumulation of the nonmethylated anthocyanin delphinidin 3-rutinoside. The coexpression of PAP1 and AOMT resulted in an accumulation of malvidin 3-rutinoside. We also showed that AOMT localized exclusively in the cytoplasm of tobacco leaf cells. These results demonstrate the ability of this enzyme to methylate anthocyanins both in vitro and in vivo, indicating that AOMT plays a major role in anthocyanin biosynthesis in grape berries.
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