ArticleApplied and environmental microbiology2009
Adherence of Escherichia coli O157:H7 mutants in vitro and in ligated pig intestines.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- Flavourzyme Suppresses Pseudomonas aeruginosa Biofilms by Targeting Motility, Quorum Sensing, and Virulence Genes.Current microbiology · 2025Article
- Porcine and Chicken Intestinal Epithelial Cell Models for Screening Phytogenic Feed Additives-Chances and Limitations in Use as Alternatives to Feeding Trials.Microorganisms · 2022Review
- Lactic acid bacteria that activate immune gene expression in Caenorhabditis elegans can antagonise Campylobacter jejuni infection in nematodes, chickens and mice.BMC microbiology · 2021Article
- Magnesium Sensing Regulates Intestinal Colonization of Enterohemorrhagic Escherichia coli O157:H7.mBio · 2020Article
- Lactic acid bacteria exhibit similar antioxidant capacities inRSC advances · 2020Article
- Cell Signaling ofFrontiers in immunology · 2018Article
- Transcriptional Activator GmrA, Encoded in Genomic Island OI-29, Controls the Motility of EnterohemorrhagicFrontiers in microbiology · 2018Article
- Alteration of the Microbiota and Virulence Gene Expression in E. coli O157:H7 in Pig Ligated Intestine with and without AE Lesions.PloS one · 2015Article
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- An O island 172 encoded RNA helicase regulates the motility of Escherichia coli O157:H7.PloS one · 2013Article
- Porcine IPEC-J2 intestinal epithelial cells in microbiological investigations.Veterinary microbiology · 2012Review
- Involvement of quorum sensing and heat-stable enterotoxin a in cell damage caused by a porcine enterotoxigenic Escherichia coli strain.Infection and immunity · 2011Article
- Adherence of Escherichia coli O157:H7 to epithelial cells in vitro and in pig gut loops is affected by bacterial culture conditions.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2011Article
- Differential gene expression and adherence of Escherichia coli O157:H7 in vitro and in ligated pig intestines.PloS one · 2011Article
- Enhanced Actin Pedestal Formation by Enterohemorrhagic Escherichia coli O157:H7 Adapted to the Mammalian Host.Frontiers in microbiology · 2011Article
- Neutralizing antibodies to Shiga toxin type 2 (Stx2) reduce colonization of mice by Stx2-expressing Escherichia coli O157:H7.Vaccine · 2010Article
- Verotoxin 2 enhances adherence of enterohemorrhagic Escherichia coli O157:H7 to intestinal epithelial cells and expression of {beta}1-integrin by IPEC-J2 cells.Applied and environmental microbiology · 2010Article
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Corrections and comments
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Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There are contradictory literature reports on the role of verotoxin (VT) in adherence of enterohemorrhagic Escherichia coli O157:H7 (O157 EHEC) to intestinal epithelium. There are reports that putative virulence genes of O island 7 (OI-7), OI-15, and OI-48 of this pathogen may also affect adherence in vitro. Therefore, mutants of vt2 and segments of OI-7 and genes aidA(15) (gene from OI-15) and aidA(48) (gene from OI-48) were generated and evaluated for adherence in vitro to cultured human HEp-2 and porcine jejunal epithelial (IPEC-J2) cells and in vivo to enterocytes in pig ileal loops. VT2-negative mutants showed significant decreases in adherence to both HEp-2 and IPEC-J2 cells and to enterocytes in pig ileal loops; complementation only partially restored VT2 production but fully restored the adherence to the wild-type level on cultured cells. Deletion of OI-7 and aidA(48) had no effect on adherence, whereas deletion of aidA(15) resulted in a significant decrease in adherence in pig ileal loops but not to the cultured cells. This investigation supports the findings that VT2 plays a role in adherence, shows that results obtained in adherence of E. coli O157:H7 in vivo may differ from those obtained in vitro, and identified AIDA-15 as having a role in adherence of E. coli O157:H7.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.