ArticleThe Journal of biological chemistry2009
Nuclear export of the yeast hexokinase 2 protein requires the Xpo1 (Crm1)-dependent pathway.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Multilayered control of hexose uptake and phosphorylation through specialized sugar kinases in Nakaseomyces glabratus.Fungal biology and biotechnology · 2026Article
- Changing course: Glucose starvation drives nuclear accumulation of Hexokinase 2 in S. cerevisiae.PLoS genetics · 2023Article
- Proteomic consequences of TDA1 deficiency in Saccharomyces cerevisiae: Protein kinase Tda1 is essential for Hxk1 and Hxk2 serine 15 phosphorylation.Scientific reports · 2022Article
- Article
- Moonlighting Proteins: The Case of the Hexokinases.Frontiers in molecular biosciences · 2021Review
- Hexokinase 2 and nuclear factor erythroid 2-related factor 2 transcriptionally coactivate xanthine oxidoreductase expression in stressed glioma cells.The Journal of biological chemistry · 2018Article
- Hexokinase 2 Is an Intracellular Glucose Sensor of Yeast Cells That Maintains the Structure and Activity of Mig1 Protein Repressor Complex.The Journal of biological chemistry · 2016Article
- Protein kinase Ymr291w/Tda1 is essential for glucose signaling in saccharomyces cerevisiae on the level of hexokinase isoenzyme ScHxk2 phosphorylation*.The Journal of biological chemistry · 2015Article
- Cancer stem cell theory and the warburg effect, two sides of the same coin?International journal of molecular sciences · 2014Article
- Proteomic and functional consequences of hexokinase deficiency in glucose-repressible Kluyveromyces lactis.Molecular & cellular proteomics : MCP · 2014Article
- Article
- In scarcity and abundance: metabolic signals regulating cell growth.Physiology (Bethesda, Md.) · 2013Review
- Phosphorylation of yeast hexokinase 2 regulates its nucleocytoplasmic shuttling.The Journal of biological chemistry · 2012Article
- Yeast importin-β is required for nuclear import of the Mig2 repressor.BMC cell biology · 2012Article
- The yeast nuclear pore complex and transport through it.Genetics · 2012Article
- Nuclear import of the yeast hexokinase 2 protein requires α/β-importin-dependent pathway.The Journal of biological chemistry · 2012Article
- Crystal structure of hexokinase KlHxk1 of Kluyveromyces lactis: a molecular basis for understanding the control of yeast hexokinase functions via covalent modification and oligomerization.The Journal of biological chemistry · 2010Article
- Functional domains of yeast hexokinase 2.The Biochemical journal · 2010Article
- Nucleocytoplasmic shuttling of hexokinase II in a cancer cell.Biochemical and biophysical research communications · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hexokinase 2 (Hxk2) from Saccharomyces cerevisiae was one of the first metabolic enzymes described as a multifunctional protein. Hxk2 has a double subcellular localization; it functions as a glycolytic enzyme in the cytoplasm and as a regulator of gene transcription of several Mig1-regulated genes in the nucleus. However, the mechanism by which Hxk2 enters and leaves the nucleus is still unknown. In low glucose conditions, Hxk2 is phosphorylated at serine 14, but how this phosphorylation may affect glucose signaling is also unknown at the moment. Here we report that the Hxk2 protein is an export substrate of the carrier protein Xpo1 (Crm1). We also show that the Hxk2 nuclear export and the binding of Hxk2 and Xpo1 involve two leucine-rich nuclear export signals (NES) located between leucine 23 and isoleucine 33 (NES1) and between leucine 310 and leucine 318 (NES2). We also show that the Hxk2 phosphorylation at serine 14 promotes Hxk2 export by facilitating the association of Hxk2 with Xpo1. Our study uncovers a new cargo for the Xpo1 yeast exportin and identifies Hxk2 phosphorylation at serine 14 as a regulatory mechanism that controls its nuclear exit in function of the glucose levels.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.