ReviewJournal of neuroscience methods2009
Establishing a physiological environment for visualized in vitro brain slice recordings by increasing oxygen supply and modifying aCSF content.
Review in Journal of neuroscience methods, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
63 citing papers in PubMed.
- Localized delivery of hyaluronic acid-doxorubicin from a surgical paste for post-operative glioblastoma treatment.Materials today. Bio · 2026Article
- Artificial cerebrospinal fluid preserves neuronal viability and attenuates apoptosis and oxidative stress in HT22 cells.Frontiers in pharmacology · 2026Article
- From mice to clinical relevance: humanizing neuroscience with human-based model systems.Frontiers in cellular neuroscience · 2026Review
- Review
- Trauma in Neonatal Acute Brain Slices Alters Calcium and Network Dynamics and Causes Calpain-Mediated Cell Death.eNeuro · 2024Article
- Microglia contribute to neuronal synchrony despite endogenous ATP-related phenotypic transformation in acute mouse brain slices.Nature communications · 2024Article
- Time-dependent phenotypical changes of microglia drive alterations in hippocampal synaptic transmission in acute slices.Frontiers in cellular neuroscience · 2024Article
- Temporal disparity of action potentials triggered in axon initial segments and distal axons in the neocortex.Science advances · 2023Article
- Alterations of sleep oscillations in Alzheimer's disease: A potential role for GABAergic neurons in the cortex, hippocampus, and thalamus.Brain research bulletin · 2022Review
- Alternative Brain Slice-on-a-Chip for Organotypic Culture and Effective Fluorescence Injection Testing.International journal of molecular sciences · 2022Article
- Ascorbic Acid Reduces Neurotransmission, Synaptic Plasticity, and Spontaneous Hippocampal Rhythms in In Vitro Slices.Nutrients · 2022Article
- Artificial sleep-like up/down-states induce synaptic plasticity in cortical neurons from mouse brain slices.Frontiers in cellular neuroscience · 2022Article
- Vulnerability of cholecystokinin-expressing GABAergic interneurons in the unilateral intrahippocampal kainate mouse model of temporal lobe epilepsy.Experimental neurology · 2021Article
- Epileptiform activity in mouse hippocampal slices induced by moderate changes in extracellular MgBMC neuroscience · 2021Article
- Sharp Wave Ripples in Alzheimer's Disease: In Search of Mechanisms.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Impaired State-Dependent Potentiation of GABAergic Synaptic Currents Triggers Seizures in a Genetic Generalized Epilepsy Model.Cerebral cortex (New York, N.Y. : 1991) · 2021Article
- Electrographic Features of Spontaneous Recurrent Seizures in a Mouse Model of Extended Hippocampal Kindling.Cerebral cortex communications · 2021Article
- New Insights and Methods for Recording and Imaging Spontaneous Spreading Depolarizations and Seizure-Like Events in Mouse Hippocampal Slices.Frontiers in cellular neuroscience · 2021Article
- Examinations of Bilateral Epileptiform Activities in Hippocampal Slices Obtained From Young Mice.Frontiers in cellular neuroscience · 2020Article
- Understanding the Effects of General Anesthetics on Cortical Network Activity Using Ex Vivo Preparations.Anesthesiology · 2019Review
3 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors.
Funding
Abstract
Our insights into the basic characteristics of neuronal function were significantly advanced by combining the in vitro slice technique with the visualization of neurons and their processes. The visualization through water immersion objectives requires keeping slices submerged in recording chambers where delivering artificial cerebro-spinal fluid (aCSF) at flow rates of 2-3 ml/min results in a limited oxygen supply [Hájos N, Ellender TJ, Zemankovics R, Mann EO, Exley R, Cragg SJ, et al. Maintaining network activity in submerged hippocampal slices: importance of oxygen supply. Eur J Neurosci 2009;29:319-27]. Here we review two methods aimed at providing sufficient oxygen levels to neurons in submerged slices to enable high energy consuming processes such as elevated firing rates or network oscillations. The use of these methods may also influence the outcome of other electrophysiological experiments in submerged slices including the study of intercellular signaling pathways. In addition, we also emphasize the importance of various aCSF constituents used in in vitro experiments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.