ArticleJournal of immunological methods2009
Numbers matter: quantitative and dynamic analysis of the formation of an immunological synapse using imaging flow cytometry.
Article in Journal of immunological methods, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- Explainable machine learning for profiling the immunological synapse and functional characterization of therapeutic antibodies.Nature communications · 2023Article
- Impact of Mycobacterium tuberculosis Glycolipids on the CD4+ T Cell-Macrophage Immunological Synapse.Journal of immunology (Baltimore, Md. : 1950) · 2023Article
- Bioengineering tools for probing intracellular events in T lymphocytes.WIREs mechanisms of disease · 2021Review
- Antigen-independent activation enhances the efficacy of 4-1BB-costimulated CD22 CAR T cells.Nature medicine · 2021Article
- High-throughput analysis of T cell-monocyte interaction in human tuberculosis.Clinical and experimental immunology · 2020Article
- CXCR3 chemokine receptor contributes to specific CD8+ T cell activation by pDC during infection with intracellular pathogens.PLoS neglected tropical diseases · 2020Article
- T cells transduce T-cell receptor signal strength by generating different phosphatidylinositols.The Journal of biological chemistry · 2019Article
- Polarity of CD4+ T cells towards the antigen presenting cell is regulated by the Lck adapter TSAd.Scientific reports · 2018Article
- In vitro analysis of antigen induced T cell-monocyte conjugates by imaging flow cytometry.Journal of immunological methods · 2018Article
- Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry.Journal of visualized experiments : JoVE · 2017Article
- Masks in imaging flow cytometry.Methods (San Diego, Calif.) · 2017Review
- Anti-TNF drives regulatory T cell expansion by paradoxically promoting membrane TNF-TNF-RII binding in rheumatoid arthritis.The Journal of experimental medicine · 2016Article
- Quantification of CD4(+) T Cell Alloreactivity and Its Control by Regulatory T Cells Using Time-Lapse Microscopy and Immune Synapse Detection.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2016Article
- Imaging flow cytometry: coping with heterogeneity in biological systems.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2012Review
- Analyzing cellular internalization of nanoparticles and bacteria by multi-spectral imaging flow cytometry.Journal of visualized experiments : JoVE · 2012Article
- Visualization and quantitation of the expression of microRNAs and their target genes in neuroblastoma single cells using imaging cytometry.BMC research notes · 2011Article
- Single cell force spectroscopy of T cells recognizing a myelin-derived peptide on antigen presenting cells.Immunology letters · 2011Article
- Imaging techniques for assaying lymphocyte activation in action.Nature reviews. Immunology · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Activation of T lymphocytes by antigen-presenting cells (APC) results in the formation of an immunological synapse. Following contact with the target cell, key signaling and adhesion molecules polarize within minutes to hours to the T cell-APC interface. Multispectral imaging flow cytometry, a new technology which combines flow cytometry with imaging, was used to visualize and quantify the recruitment of the CD3epsilon and Lck signaling molecules during the evolution of an immune synapse. Using this technology, thousands of T cell/macrophage conjugates could be analyzed for each experimental time point. Following Ca++ triggered T cell activation, the dynamics of Lck and CD3epsilon recruitment to the synapse, analyzed by two independent methods, were comparable. However, CD3epsilon exhibited longer residence times (>8 min) at the synapse than Lck.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.