ArticleVaccine2009
Altered pathogenicity, immunogenicity, tissue tropism and 3'-7kb region sequence of an avian infectious bronchitis coronavirus strain after serial passage in embryos.
Article in Vaccine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 47 citations in OpenAlex.
- Article
- Seroprevalence of Infectious Bronchitis Virus Antibodies in Vaccinated Broilers from South-Western Romania (2018-2021): An ELISA-Based Survey.Veterinary sciences · 2026Article
- Attenuation and altered replication of IBV strain tl/CH/LDT3/03 after serial passage in chicken embryo fibroblasts and Vero cells.Virus research · 2025Article
- Multi-Organ Spread and Intra-Host Diversity of SARS-CoV-2 Support Viral Persistence, Adaptation, and a Mechanism That Increases Evolvability.Journal of medical virology · 2024Article
- Novel recombinant avian infectious bronchitis viruses from chickens in Korea, 2019-2021.Frontiers in veterinary science · 2023Article
- Molecular Characterization Analysis of Prevalent Infectious Bronchitis Virus and Pathogenicity Assessment of Recombination Strain in China.Frontiers in veterinary science · 2022Article
- Innate immune responses of domestic pigeons to the infection of pigeon paramyxovirus type 1 virus.Poultry science · 2021Article
- Genetic and antigenic heterogeneity of GI-1/Massachusetts lineage infectious bronchitis virus variants recently isolated in China.Poultry science · 2020Article
- Might SARS-CoV-2 Have Arisen via Serial Passage through an Animal Host or Cell Culture?: A potential explanation for much of the novel coronavirus' distinctive genome.BioEssays : news and reviews in molecular, cellular and developmental biology · 2020Article
- A highly pathogenic GI-19 lineage infectious bronchitis virus originated from multiple recombination events with broad tissue tropism.Virus research · 2020Article
- Genetic sequence changes related to the attenuation of avian infectious bronchitis virus strain TW2575/98.Virus genes · 2020Article
- Genetic, antigenic and pathogenic characterization of avian coronaviruses isolated from pheasants (Phasianus colchicus) in China.Veterinary microbiology · 2020Article
- Genetic and biological characteristics of four novel recombinant avian infectious bronchitis viruses isolated in China.Virus research · 2019Article
- Novel genotype of infectious bronchitis virus isolated in China.Veterinary microbiology · 2019Article
- Serotype, antigenicity, and pathogenicity of a naturally recombinant TW I genotype infectious bronchitis coronavirus in China.Veterinary microbiology · 2016Article
- Recombinant duck enteritis viruses expressing major structural proteins of the infectious bronchitis virus provide protection against infectious bronchitis in chickens.Antiviral research · 2016Article
- Molecular and antigenic characteristics of Massachusetts genotype infectious bronchitis coronavirus in China.Veterinary microbiology · 2015Article
- Differential modulation of avian β-defensin and Toll-like receptor expression in chickens infected with infectious bronchitis virus.Applied microbiology and biotechnology · 2015Article
- Serotype shift of a 793/B genotype infectious bronchitis coronavirus by natural recombination.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2015Article
- Development and efficacy of a novel live-attenuated QX-like nephropathogenic infectious bronchitis virus vaccine in China.Vaccine · 2015Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, we attenuated a Chinese LX4-type nephropathogenic infectious bronchitis virus (IBV) strain, CK/CH/LHLJ/04V, by serial passage in embryonated chicken eggs. Based on sequence analysis of the 3'-7kb region, the CK/CH/LHLJ/04V virus population contained subpopulations with a mixture of genetic mutants. The titers of the virus increased gradually during serial passage, but the replication capacity decreased in chickens. The virus was partially attenuated at passage 40 (P40) and P70, and was fully attenuated at P110. It lost immunogenicity and kidney tropism at P110 and P70, respectively. Amino acid substitutions were found in the 3'-7kb region, primarily in the spike (S) protein. Substitutions in the S1 subunit occurred between P3 and P40 and all subpopulations in a virus passage showed the same substitutions. Other substitutions that occurred between P70 and P110, however, were found only in some subpopulations of the virus passages. A 109-bp deletion in the 3'-UTR was observed in most subpopulations of P70 and P110, and might be related to virus replication, transcription and pathogenicity. The changes described in the 3'-7kb region of the virus are possibly responsible for virus attenuation, immunogenicity decrease and tissue tropism changes; however, we cannot exclude the possibility that other parts of the genome may also be involved in those changes.
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