ArticleMolecular plant pathology2009
Next-generation sequencing and metagenomic analysis: a universal diagnostic tool in plant virology.
Article in Molecular plant pathology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 128 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
128 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Biological and genetic characteristics of Pospiviroid exocortiscitri and Hostuviroid impedihumuli in mulberry trees from Shiraz, Iran, using infectious clones.Scientific reports · 2026Article
- Discovery of a novel emaravirus and an alphacytorhabdovirus infecting Spiraea in the USA.Archives of virology · 2026Article
- Metatranscriptomics analysis reveals the cotton virome in the southern United States.Scientific reports · 2026Article
- Pepino Mosaic Virus: A Globally Important Tomato Pathogen and a Rising Model in Molecular Virology.Molecular plant pathology · 2026Review
- Monitoring Alien Species Diversity in Ballast Water Based on Environmental DNA Metabarcoding.Ecology and evolution · 2025Article
- Recent advances in characterization and diagnosis of stone fruit tree viruses.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- High-Throughput Sequencing Reveals Apple Virome Diversity and Novel Viruses in the Czech Republic.Viruses · 2025Article
- Detection and In Vivo Validation ofViruses · 2025Article
- In-depth analysis of 17,115 rice transcriptomes reveals extensive viral diversity in rice plants.Nature communications · 2025Article
- Discovery of novel 'sugarcane totivirus 1' from Saccharum officinarum by high-throughput sequencing.Molecular biology reports · 2025Article
- High-Throughput Sequencing Detects a Viral Complex inAdvances in virology · 2025Article
- Effects of compound fermentation withFrontiers in veterinary science · 2025Article
- Comprehensive virome profiling of sugarcane and simplified duplex OneStep RT-PCR assay reveals the prevalence of sugarcane streak mosaic virus along with sugarcane yellow leaf virus in India.Journal, genetic engineering & biotechnology · 2024Article
- High-Throughput Sequencing Identified Multiple Fig Viruses and Viroids Associated with Fig Mosaic Disease in Iraq.The plant pathology journal · 2024Article
- Comprehensive Metatranscriptomic Analysis of Plant Viruses in Imported Frozen Cherries and Blueberries.The plant pathology journal · 2024Article
- Article
- Unleashing the power of colloidal gold immunochromatographic assays for plant virus diagnostics.MethodsX · 2024Review
- An evaluation of nucleic acid-based molecular methods for the detection of plant viruses: a systematic review.Virusdisease · 2024Review
- Whole genome sequence analysis of shallot virus X from India reveals it to be a natural recombinant with positive selection pressure.BMC genomic data · 2024Article
68 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel, unbiased approach to plant viral disease diagnosis has been developed which requires no a priori knowledge of the host or pathogen. Next-generation sequencing coupled with metagenomic analysis was used to produce large quantities of cDNA sequence in a model system of tomato infected with Pepino mosaic virus. The method was then applied to a sample of Gomphrena globosa infected with an unknown pathogen originally isolated from the flowering plant Liatris spicata. This plant was found to contain a new cucumovirus, for which we suggest the name 'Gayfeather mild mottle virus'. In both cases, the full viral genome was sequenced. This method expedites the entire process of novel virus discovery, identification, viral genome sequencing and, subsequently, the development of more routine assays for new viral pathogens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.