ArticleMolecular biotechnology2009
cDNA cloning and functional expression of the alpha-D-galactose-binding lectin frutalin in Escherichia coli.
Article in Molecular biotechnology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- Cytotoxicity of Frutalin on Distinct Cancer Cells Is Independent of Its Glycosylation.Molecules (Basel, Switzerland) · 2021Article
- Expression of Lectins in Heterologous Systems.International journal of molecular sciences · 2018Review
- Functional Expression and Characterization of the Recombinant N-Acetyl-Glucosamine/N-Acetyl-Galactosamine-Specific Marine Algal Lectin BPL3.Marine drugs · 2018Article
- Frutapin, a lectin fromBioscience reports · 2017Article
- Anti-Neuroblastoma Properties of a Recombinant Sunflower Lectin.International journal of molecular sciences · 2017Article
- Label-Free Proteome Analysis of Plasma from Patients with Breast Cancer: Stage-Specific Protein Expression.Frontiers in oncology · 2017Article
- Crystallization and preliminary X-ray diffraction studies of frutalin, an α-D-galactose-specific lectin from Artocarpus incisa seeds.Acta crystallographica. Section F, Structural biology communications · 2015Article
- Functional recombinants designed from a fetuin/asialofetuin-specific marine algal lectin, rhodobindin.Marine drugs · 2015Article
- Recombinant production of plant lectins in microbial systems for biomedical application - the frutalin case study.Frontiers in plant science · 2014Review
- Fusion tags for protein solubility, purification and immunogenicity in Escherichia coli: the novel Fh8 system.Frontiers in microbiology · 2014Review
- Effect of the lectin of Bauhinia variegata and its recombinant isoform on surgically induced skin wounds in a murine model.Molecules (Basel, Switzerland) · 2011Article
- Influence of induction conditions on the expression of carbazole dioxygenase components (CarAa, CarAc, and CarAd) from Pseudomonas stutzeri in recombinant Escherichia coli using experimental design.Journal of industrial microbiology & biotechnology · 2011Article
- Cytotoxic effects of native and recombinant frutalin, a plant galactose-binding lectin, on HeLa cervical cancer cells.Journal of biomedicine & biotechnology · 2011Article
- Lectins: production and practical applications.Applied microbiology and biotechnology · 2011Review
- Bridging the gap: a GFP-based strategy for overexpression and purification of membrane proteins with intra and extracellular C-termini.Protein science : a publication of the Protein Society · 2010Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
cDNA clones encoding frutalin, the alpha-D-galactose-binding lectin expressed in breadfruit seeds (Artocarpus incisa), were isolated and sequenced. The deduced amino acid sequences indicated that frutalin may be encoded by a family of genes. The NCBI database searches revealed that the frutalin sequence is highly homologous with jacalin and mornigaG sequences. Frutalin cDNA was re-amplified and cloned into the commercial expression vector pET-25b(+) for frutalin production in Escherichia coli. An experimental factorial design was employed to maximise the soluble expression of the recombinant lectin. The results indicated that temperature, time of induction, concentration of IPTG and the interaction between the concentration of IPTG and the time of induction had the most significant effects on the soluble expression level of recombinant frutalin. The optimal culture conditions were as follows: induction with 1 mM IPTG at 22 degrees C for 20 h, yielding 16 mg/l of soluble recombinant frutalin. SDS-PAGE and Western blot analysis revealed that recombinant frutalin was successfully expressed by bacteria with the expected molecular weight (17 kDa). These analyses also showed that recombinant frutalin was mainly produced as insoluble protein. Recombinant frutalin produced by bacteria revealed agglutination properties and carbohydrate-binding specificity similar to the native breadfruit lectin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.