ArticleJournal of industrial microbiology & biotechnology2009
Chemical induction of silent biosynthetic pathway transcription in Aspergillus niger.
Article in Journal of industrial microbiology & biotechnology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed, 166 citations in OpenAlex.
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- Revolutionizing microbial treasure troves: innovative strategies for natural products discovery.Natural products and bioprospecting · 2026Review
- Global survey of secondary metabolism inPNAS nexus · 2025Article
- Unlocking hidden treasures: the evolution of high-throughput mass spectrometry in screening for cryptic natural products.Natural product reports · 2025Review
- The hidden treasures in endophytic fungi: a comprehensive review on the diversity of fungal bioactive metabolites, usual analytical methodologies, and applications.Archives of microbiology · 2024Review
- Chemical Epigenetic Regulation Secondary Metabolites Derived fromMolecules (Basel, Switzerland) · 2024Article
- Recent Advances in Search of Bioactive Secondary Metabolites from Fungi Triggered by Chemical Epigenetic Modifiers.Journal of fungi (Basel, Switzerland) · 2023Review
- Epigenetic manipulation for secondary metabolite activation in endophytic fungi: current progress and future directions.Mycology · 2023Review
- Metabolomic Strategy to Characterize the Profile of Secondary Metabolites inMolecules (Basel, Switzerland) · 2022Article
- Complementary Strategies to Unlock Biosynthesis Gene Clusters Encoding Secondary Metabolites in the Filamentous FungusJournal of fungi (Basel, Switzerland) · 2022Article
- A histone H3K9 methyltransferase Dim5 mediates repression of sorbicillinoid biosynthesis in Trichoderma reesei.Microbial biotechnology · 2022Article
- Induction of Secondary Metabolite Biosynthesis by Deleting the Histone Deacetylase HdaA in the Marine-Derived FungusJournal of fungi (Basel, Switzerland) · 2022Article
- Post-translational modifications drive secondary metabolite biosynthesis in Aspergillus: a review.Environmental microbiology · 2022Review
- Cryptic Metabolites from Marine-Derived Microorganisms Using OSMAC and Epigenetic Approaches.Marine drugs · 2022Review
- Epigenetic Modification: A Key Tool for Secondary Metabolite Production in Microorganisms.Frontiers in microbiology · 2022Review
- Fungal Endophytes: A Promising Frontier for Discovery of Novel Bioactive Compounds.Journal of fungi (Basel, Switzerland) · 2021Review
- Investigating the Role of Vanadium-Dependent Haloperoxidase Enzymology in Microbial Secondary Metabolism and Chemical Ecology.mSystems · 2021Article
- Article
- Secondary metabolites from hypocrealean entomopathogenic fungi: novel bioactive compounds.Natural product reports · 2020Review
- Epigenetic modulation of secondary metabolite profiles in Aspergillus calidoustus and Aspergillus westerdijkiae through histone deacetylase (HDAC) inhibition by vorinostat.The Journal of antibiotics · 2020Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Manipulation of the fungal epigenome is hypothesized to be an effective method for accessing natural products from silent biosynthetic pathways. A library of epigenetic modifiers was tested using the fungus Aspergillus niger to determine the impact of small-molecule inhibitors on reversing the transcriptional suppression of biosynthetic genes involved in polyketide (PKS), non-ribosomal peptide (NRPS), and hybrid PKS-NRPS (HPN) production. Examination of expressed sequence tag libraries from A. niger demonstrated that >70% of its PKS-, NRPS-, and HPN-encoding gene clusters were transcriptionally suppressed under standard laboratory culture conditions. Using a chemical epigenetic methodology, we showed that treatment of A. niger with suberoylanilide hydroxamic acid and 5-azacytidine led to the transcriptional upregulation of many secondary-metabolite-encoding biosynthetic gene clusters. Chemical epigenetic modifiers exhibited positional biases for upregulating chromosomally distal gene clusters. In addition, a phylogenetic-based preference was noted in the upregulation of reducing clade I PKS gene clusters, while reducing clade IV PKS gene clusters were largely unaffected. Manipulating epigenetic features in fungi is a powerful method for accessing the products of silent biosynthetic pathways. Moreover, this approach can be readily incorporated into modern microbial screening operations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.