ArticleFrontiers in neural circuits2009
Live imaging of synaptic vesicle release and retrieval in dopaminergic neurons.
Article in Frontiers in neural circuits, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 44 citations in OpenAlex.
- The landscape of multiscale transcriptomic networks and key regulators in Parkinson's disease.Nature communications · 2019Pooled it
- Regulation of Dopamine Release by Tonic Activity Patterns in the Striatal Brain Slice.ACS chemical neuroscience · 2025Article
- Article
- Selective dopaminergic vulnerability in Parkinson's disease: new insights into the role of DAT.Frontiers in neuroscience · 2023Review
- A fluorescent nanosensor paint detects dopamine release at axonal varicosities with high spatiotemporal resolution.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Opponent vesicular transporters regulate the strength of glutamatergic neurotransmission in a C. elegans sensory circuit.Nature communications · 2021Article
- A dopaminergic mechanism of antipsychotic drug efficacy, failure, and failure reversal: the role of the dopamine transporter.Molecular psychiatry · 2020Article
- Synj1 haploinsufficiency causes dopamine neuron vulnerability and alpha-synuclein accumulation in mice.Human molecular genetics · 2020Article
- Cyclization step of noradrenaline and adrenaline autoxidation: a quantum chemical study.RSC advances · 2020Article
- Dopamine Autoxidation Is Controlled by Acidic pH.Frontiers in molecular neuroscience · 2018Article
- Parkinson's Disease-Associated LRRK2 Hyperactive Kinase Mutant Disrupts Synaptic Vesicle Trafficking in Ventral Midbrain Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Unique pH dynamics in GABAergic synaptic vesicles illuminates the mechanism and kinetics of GABA loading.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Striatal dopamine neurotransmission: regulation of release and uptake.Basal ganglia · 2016Article
- Fluorescent false neurotransmitter reveals functionally silent dopamine vesicle clusters in the striatum.Nature neuroscience · 2016Article
- VPS35 in Dopamine Neurons Is Required for Endosome-to-Golgi Retrieval of Lamp2a, a Receptor of Chaperone-Mediated Autophagy That Is Critical for α-Synuclein Degradation and Prevention of Pathogenesis of Parkinson's Disease.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Nicotine exploits a COPI-mediated process for chaperone-mediated up-regulation of its receptors.The Journal of general physiology · 2014Article
- Calbindin controls release probability in ventral tegmental area dopamine neurons.Nature neuroscience · 2012Article
- Regulation of striatal dopamine release by presynaptic auto- and heteroreceptors.Basal ganglia · 2012Article
- Dopamine release in the basal ganglia.Neuroscience · 2011Review
- Trafficking of alpha4* nicotinic receptors revealed by superecliptic phluorin: effects of a beta4 amyotrophic lateral sclerosis-associated mutation and chronic exposure to nicotine.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Dopaminergic (DA) neurons represent <0.01% of neurons in the human brain, but are essential for normal neurological and psychiatric function. The majority of these neurons reside in the ventral midbrain, but they exert their profound influences on brain function through projections to both the cortex and the basal ganglia. These projections secrete dopamine from small clear synaptic vesicles (SVs) in axonal varicosities. DA signaling has unique spatial and temporal characteristics as compared to the fast, focal synaptic transmission of excitatory and inhibitory neurons. However, as with fast-acting neurotransmitters, DA SVs must be locally recycled for use following exocytosis. Little is known about these DA SV recycling properties and how they might impact efficacy of DA neurotransmission. Here we used the pH-sensitive fluorescent probe synaptopHluorin to investigate SV recycling in DA neurons and compared their properties to prototypical fast neurotransmitter synapses of the hippocampus. These measurements showed that DA SVs, like hippocampal SVs, have a resting pH of approximately 5.6. However, compared to hippocampal neurons, DA neurons show limited depletion of the recycling pool of vesicles as the stimulus frequency is increased from 5 to 30 Hz. Additional measurements show that exocytosis rates at this frequency are comparable between hippocampal and DA neurons. Thus, limited vesicle depletion likely arises from a stimulus frequency-dependent acceleration of DA SV endocytosis or re-acidification. Our observations imply differential regulation of endocytic-exocytic balance in DA neurons. Finally, our assay can also be used to investigate the effects of genetic and chemical modulation of the SV cycle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.