ArticleThe Journal of biological chemistry2009
Degradation of sterol regulatory element-binding protein precursor requires the endoplasmic reticulum-associated degradation components Ubc7 and Hrd1 in fission yeast.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 34 citations in OpenAlex.
- Loss of the E3 ubiquitin ligase MARCHF6 alters hepatic lipid metabolism and drives spontaneous hepatosteatosis.Molecular metabolism · 2026Article
- Exogenous HJournal of cachexia, sarcopenia and muscle · 2023Article
- PIDDosome-SCAP crosstalk controls high-fructose-diet-dependent transition from simple steatosis to steatohepatitis.Cell metabolism · 2022Article
- Outline of the biosynthesis and regulation of ergosterol in yeast.World journal of microbiology & biotechnology · 2019Review
- Oxygen-responsive transcriptional regulation of lipid homeostasis in fungi: Implications for anti-fungal drug development.Seminars in cell & developmental biology · 2018Review
- Membrane Protein Quantity Control at the Endoplasmic Reticulum.The Journal of membrane biology · 2017Review
- Coordinate Regulation of Yeast Sterol Regulatory Element-binding Protein (SREBP) and Mga2 Transcription Factors.The Journal of biological chemistry · 2017Article
- Complex structure of the fission yeast SREBP-SCAP binding domains reveals an oligomeric organization.Cell research · 2016Article
- Fatostatin blocks ER exit of SCAP but inhibits cell growth in a SCAP-independent manner.Journal of lipid research · 2016Article
- Function and Regulation of Cph2 in Candida albicans.Eukaryotic cell · 2015Article
- Endoplasmic Reticulum Exit of Golgi-resident Defective for SREBP Cleavage (Dsc) E3 Ligase Complex Requires Its Activity.The Journal of biological chemistry · 2015Article
- Structure of the WD40 domain of SCAP from fission yeast reveals the molecular basis for SREBP recognition.Cell research · 2015Article
- ChIP-seq and in vivo transcriptome analyses of the Aspergillus fumigatus SREBP SrbA reveals a new regulator of the fungal hypoxia response and virulence.PLoS pathogens · 2014Article
- Structural requirements for sterol regulatory element-binding protein (SREBP) cleavage in fission yeast.The Journal of biological chemistry · 2013Article
- Induction of secondary dormancy by hypoxia in barley grains and its hormonal regulation.Journal of experimental botany · 2013Article
- Dsc orthologs are required for hypoxia adaptation, triazole drug responses, and fungal virulence in Aspergillus fumigatus.Eukaryotic cell · 2012Article
- Regulation of SREBP during hypoxia requires Ofd1-mediated control of both DNA binding and degradation.Molecular biology of the cell · 2012Article
- Identifying a static nonlinear structure in a biological system using noisy, sparse data.Journal of theoretical biology · 2012Article
- Protein quality control in the ER: balancing the ubiquitin checkbook.Trends in cell biology · 2012Review
- Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.Molecular cell · 2011Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Sre1, the fission yeast sterol regulatory element-binding protein (SREBP), is an endoplasmic reticulum (ER) membrane-bound transcription factor that is a principal regulator of hypoxic gene expression. Under low oxygen, Sre1 is cleaved from its inactive ER precursor form to generate an active nuclear transcription factor that up-regulates genes required for low oxygen growth. To maintain a constant supply of Sre1, Sre1 precursor synthesis must be regulated to replenish Sre1 precursor lost to proteolytic cleavage under low oxygen. In this study, we investigated the mechanisms controlling Sre1 precursor levels. We found that positive feedback regulation at the sre1(+) promoter increases the synthesis of the Sre1 precursor under low oxygen and that this regulation is required for maximal Sre1 activation and target gene expression. We also demonstrate that the Sre1 precursor is rapidly degraded by the proteasome in the absence of its binding partner Scp1, which is required for oxygen-regulated Sre1 cleavage. Degradation of Sre1 in the absence of Scp1 requires the ER-associated degradation (ERAD) components Ubc7, an E2 ubiquitin conjugating enzyme, and Hrd1, an E3 ubiquitin ligase. We conclude that positive feedback regulation to up-regulate Sre1 precursor synthesis under low oxygen is essential for Sre1 function and propose that excess Sre1 precursor is removed by ERAD to ensure complex formation between Sre1 and its binding partner Scp1. Thus, Sre1 is a new example of an endogenous ERAD substrate, establishing fission yeast as an organism for the study of this important degradative pathway.
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