ArticleThe Journal of biological chemistry2009
Selective binding of glutathione conjugates of fatty acid derivatives by plant glutathione transferases.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 102 citations in OpenAlex.
- The competitive interplay of 12-oxophytodienoic acid (OPDA), protein thiols, and glutathione.The FEBS journal · 2026Article
- Downstream metabolites of (+)-cis-12-oxo-phytodienoic acid function as noncanonical bioactive jasmonates in Arabidopsis thaliana.Nature communications · 2025Article
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- Jasmonic acid signaling and glutathione coordinate plant recovery from high light stress.Plant physiology · 2025Article
- Overlooked and misunderstood: can glutathione conjugates be clues to understanding plant glutathione transferases?Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- OPDA/dn-OPDA actions: biosynthesis, metabolism, and signaling.Plant cell reports · 2024Review
- ΔiScience · 2024Article
- Transcriptomic changes in barley leaves induced by alcohol ethoxylates indicate potential pathways of surfactant detoxification.Scientific reports · 2024Article
- The interaction networks of small rubber particle proteins in the latex ofFrontiers in plant science · 2024Article
- Proteomic Analysis of Proteins Related to Defense Responses inInternational journal of molecular sciences · 2023Article
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- Early loss of glutathione -s- transferase (GST) activity during diverse forms of acute renal tubular injury.Physiological reports · 2022Article
- The Phytotoxin Myrigalone A Triggers a Phased Detoxification Programme and InhibitsInternational journal of molecular sciences · 2022Article
- Glutathione-S-transferases genes-promising predictors of hepatic dysfunction.World journal of hepatology · 2021Review
- Transcriptomics Reveals Fast Changes in Salicylate and Jasmonate Signaling Pathways in Shoots of Carbonate-TolerantInternational journal of molecular sciences · 2021Article
- Role of Jasmonates, Calcium, and Glutathione in Plants to Combat Abiotic Stresses Through Precise Signaling Cascade.Frontiers in plant science · 2021Review
- A glutathione-dependent control of the indole butyric acid pathway supports Arabidopsis root system adaptation to phosphate deprivation.Journal of experimental botany · 2020Article
- Glutathione S-transferase: a versatile protein family.3 Biotech · 2020Review
- Is there a role for tau glutathione transferases in tetrapyrrole metabolism and retrograde signalling in plants?Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- Photosynthetic signalling during high light stress and recovery: targets and dynamics.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
Proteomic studies with Arabidopsis thaliana have revealed that the plant-specific Tau (U) class glutathione transferases (GSTs) are selectively retained by S-hexylglutathione affinity supports. Overexpression of members of the Arabidopsis GST superfamily in Escherichia coli showed that 25 of the complement of 28 GSTUs caused the aberrant accumulation of acylated glutathione thioesters in vivo, a perturbation that was not observed with other GST classes. Each GSTU caused a specific group of fatty acyl derivatives to accumulate, which varied in chain length (C(6) to C(18)), additional oxygen content (0 or 1), and desaturation (0 or 1). Thioesters bound tightly to recombinant GSTs (K(d) approximately 1 microm), explaining their accumulation. Transient expression of GSTUs in Nicotiana benthamiana followed by recovery by Strep-tag affinity chromatography allowed the respective plant ligands to be extracted and characterized. Again, each GST showed a distinct profile of recovered metabolites, notably glutathionylated oxophytodienoic acid and related oxygenated fatty acids. Similarly, the expression of the major Tau protein GSTU19 in the endogenous host Arabidopsis led to the selective binding of the glutathionylated oxophytodienoic acid-glutathione conjugate, with the enzyme able to catalyze the conjugation reaction. Additional ligands identified in planta included other fatty acid derivatives including divinyl ethers and glutathionylated chlorogenic acid. The strong and specific retention of various oxygenated fatty acids by each GSTU and the conservation in binding observed in the different hosts suggest that these proteins have selective roles in binding and conjugating these unstable metabolites in vivo.
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