ArticleBiochemical and biophysical research communications2009
Efficient identification of phosphatidylserine-binding proteins by ORF phage display.
Article in Biochemical and biophysical research communications, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 35 citations in OpenAlex.
- Lipid flip-flop in biological membranes: through the lens of TMEM16F.Physiological reviews · 2027Review
- Rabin8 phosphorylated by NDR2, the canine early retinal degeneration gene product, directs rhodopsin Golgi-to-cilia trafficking.Journal of cell science · 2025Article
- Galectins induced from hemocytes bridge phosphatidylserine and N-glycosylated Drpr/CED-1 receptor during dendrite pruning.Nature communications · 2024Article
- The Use of Bacteriophages in Biotechnology and Recent Insights into Proteomics.Antibiotics (Basel, Switzerland) · 2022Review
- CD36 Ectodomain Detects Apoptosis in Mammalian Cells.Molecular biotechnology · 2021Article
- Comparative Ligandomic Analysis of Human Lung Epithelial Cells Exposed to PMBiomedical and environmental sciences : BES · 2020Article
- Function-first ligandomics for ocular vascular research and drug target discovery.Experimental eye research · 2019Article
- Phosphatidylserine Asymmetry Promotes the Membrane Insertion of a Transmembrane Helix.Biophysical journal · 2019Article
- Lipid transporter TMEM24/C2CD2L is a CaProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Secretogranin III as a disease-associated ligand for antiangiogenic therapy of diabetic retinopathy.The Journal of experimental medicine · 2017Article
- Lipid transport by TMEM24 at ER-plasma membrane contacts regulates pulsatile insulin secretion.Science (New York, N.Y.) · 2017Article
- Novel topography of the Rab11-effector interaction network within a ciliary membrane targeting complex.Small GTPases · 2015Review
- Article
- Reticulocalbin-1 facilitates microglial phagocytosis.PloS one · 2015Article
- Insulin biosynthetic interaction network component, TMEM24, facilitates insulin reserve pool release.Cell reports · 2013Article
- ORF phage display to identify cellular proteins with different functions.Methods (San Diego, Calif.) · 2012Article
- Galectin-3 is a new MerTK-specific eat-me signal.Journal of cellular physiology · 2012Article
- Identification of calpain substrates by ORF phage display.Molecules (Basel, Switzerland) · 2011Article
- Identification of Hnrph3 as an autoantigen for acute anterior uveitis.Clinical immunology (Orlando, Fla.) · 2011Article
- Emerging Roles for SSeCKS/Gravin/AKAP12 in the Control of Cell Proliferation, Cancer Malignancy, and Barriergenesis.Genes & cancer · 2010Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
To efficiently elucidate the biological roles of phosphatidylserine (PS), we developed open-reading-frame (ORF) phage display to identify PS-binding proteins. The procedure of phage panning was optimized with a phage clone expressing MFG-E8, a well-known PS-binding protein. Three rounds of phage panning with ORF phage display cDNA library resulted in approximately 300-fold enrichment in PS-binding activity. A total of 17 PS-binding phage clones were identified. Unlike phage display with conventional cDNA libraries, all 17 PS-binding clones were ORFs encoding 13 real proteins. Sequence analysis revealed that all identified PS-specific phage clones had dimeric basic amino acid residues. GST fusion proteins were expressed for 3 PS-binding proteins and verified for their binding activity to PS liposomes, but not phosphatidylcholine liposomes. These results elucidated previously unknown PS-binding proteins and demonstrated that ORF phage display is a versatile technology capable of efficiently identifying binding proteins for non-protein molecules like PS.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.