ArticleJournal of neurochemistry2009
Interaction of catechol and non-catechol substrates with externally or internally facing dopamine transporters.
Article in Journal of neurochemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Hypocretin receptor 1 blockade early in abstinence prevents incubation of cocaine seeking and normalizes dopamine transmission.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2026Article
- Cholesterol modulation of interactions between psychostimulants and dopamine transporters.Advances in pharmacology (San Diego, Calif.) · 2024Article
- Hypocretin / Orexin Receptor 1 Knockdown in GABA or Dopamine Neurons in the Ventral Tegmental Area Differentially Impact Mesolimbic Dopamine and Motivation for Cocaine.Addiction neuroscience · 2023Article
- Determining Ligand and Ion-Induced Conformational Changes in Serotonin Transporter with Its Fluorescent Substrates.International journal of molecular sciences · 2022Article
- Synaptic ZnTranslational psychiatry · 2021Article
- Dopamine transporter function fluctuates across sleep/wake state: potential impact for addiction.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2021Article
- Functional properties of dopamine transporter oligomers after copper linking.Journal of neurochemistry · 2018Article
- Susceptibility to traumatic stress sensitizes the dopaminergic response to cocaine and increases motivation for cocaine.Neuropharmacology · 2017Article
- Hypocretin receptor 1 blockade produces bimodal modulation of cocaine-associated mesolimbic dopamine signaling.Psychopharmacology · 2017Article
- Pharmacological characterization of a high-affinity p-tyramine transporter in rat brain synaptosomes.Scientific reports · 2016Article
- Impact of disruption of secondary binding site S2 on dopamine transporter function.Journal of neurochemistry · 2016Review
- Dopamine transporter oligomerization: impact of combining protomers with differential cocaine analog binding affinities.Journal of neurochemistry · 2015Article
- An N-terminal threonine mutation produces an efflux-favorable, sodium-primed conformation of the human dopamine transporter.Molecular pharmacology · 2014Article
- Importance of cholesterol in dopamine transporter function.Journal of neurochemistry · 2012Article
- In vivo effects of amphetamine analogs reveal evidence for serotonergic inhibition of mesolimbic dopamine transmission in the rat.The Journal of pharmacology and experimental therapeutics · 2011Article
- Site-directed mutations near transmembrane domain 1 alter conformation and function of norepinephrine and dopamine transporters.Molecular pharmacology · 2011Article
- Cocaine-insensitive dopamine transporters with intact substrate transport produced by self-administration.Biological psychiatry · 2011Article
- Article
- Membrane cholesterol modulates the outward facing conformation of the dopamine transporter and alters cocaine binding.The Journal of biological chemistry · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Our previous work suggested that collapsing the Na(+) gradient and membrane potential converts the dopamine (DA) transporter (DAT) to an inward-facing conformation with a different substrate binding profile. Here, DAT expressing human embryonic kidney 293 cells were permeabilized with digitonin, disrupting ion/voltage gradients and allowing passage of DAT substrates. The potency of p-tyramine and other non-catechols (d-amphetamine, beta-phenethylamine, MPP(+)) in inhibiting cocaine analog binding to DAT in digitonin-treated cells was markedly weakened to a level similar to that observed in cell-free membranes. In contrast, the potency of DA and another catechol, norepinephrine, was not significantly changed by the same treatment, whereas epinephrine showed only a modest reduction. These findings suggest that catechol substrates interact symmetrically with both sides of DAT and non-catechol substrates, favoring binding to outward-facing transporter. In the cocaine analog binding assay, the mutant W84L displayed enhanced intrinsic binding affinity for substrates in interacting with both outward- and inward-facing states; D313N showed wild-type-like symmetric binding; but D267L and E428Q showed an apparent improvement in the permeation pathway from the external face towards the substrate site. Thus, the structure of both substrate and transporter play a role in the sidedness and mode of interaction between them.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.