ArticleAnalytical chemistry2009
Chemoselective 15N tag for sensitive and high-resolution nuclear magnetic resonance profiling of the carboxyl-containing metabolome.
Article in Analytical chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Metabolomics: An Emerging "Omics" Platform for Systems Biology and Its Implications for Huntington Disease Research.Metabolites · 2023Review
- Multiplatform untargeted metabolomics.Magnetic resonance in chemistry : MRC · 2023Review
- Quantitative NMR Methods in Metabolomics.Handbook of experimental pharmacology · 2023Article
- Practical Aspects of NMR-Based Metabolomics.Handbook of experimental pharmacology · 2023Article
- NMR-based isotope editing, chemoselection and isotopomer distribution analysis in stable isotope resolved metabolomics.Methods (San Diego, Calif.) · 2022Review
- NMR Analysis of Carboxylate Isotopomers ofAnalytical chemistry · 2021Article
- NMR-Based Metabolomics.Advances in experimental medicine and biology · 2021Article
- NMR as a "Gold Standard" Method in Drug Design and Discovery.Molecules (Basel, Switzerland) · 2020Review
- NMR Spectroscopy for Metabolomics Research.Metabolites · 2019Review
- Article
- Applications of NMR spectroscopy to systems biochemistry.Progress in nuclear magnetic resonance spectroscopy · 2016Review
- Can NMR solve some significant challenges in metabolomics?Journal of magnetic resonance (San Diego, Calif. : 1997) · 2015Review
- Chemoselective detection and discrimination of carbonyl-containing compounds in metabolite mixtures by 1H-detected 15N nuclear magnetic resonance.Magnetic resonance in chemistry : MRC · 2015Article
- Quantitating metabolites in protein precipitated serum using NMR spectroscopy.Analytical chemistry · 2014Article
- Multidimensional approaches to NMR-based metabolomics.Analytical chemistry · 2014Review
- Development and in silico evaluation of large-scale metabolite identification methods using functional group detection for metabolomics.Frontiers in genetics · 2014Article
- 15N-cholamine--a smart isotope tag for combining NMR- and MS-based metabolite profiling.Analytical chemistry · 2013Article
- Combining Hydrophilic Interaction Chromatography (HILIC) and Isotope Tagging for Off-Line LC-NMR Applications in Metabolite Analysis.Metabolites · 2013Article
- Metabolic Profiling of Green Tea Treatments in Zucker Diabetic Rats UsingJournal of nutrition & food sciences · 2013Article
- Metabolic profiling: are we en route to better diagnostic tests for cancer?Future oncology (London, England) · 2012Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Metabolic profiling has received increasing recognition as an indispensable complement to genomics and proteomics for probing biological systems and for clinical applications. (1)H nuclear magnetic resonance (NMR) is widely used in the field but is challenged by spectral complexity and overlap. Improved and simple methods that quantitatively profile a large number of metabolites are sought to make further progress. Here, we demonstrate a simple isotope tagging strategy, in which metabolites with carboxyl groups are chemically tagged with (15)N-ethanolamine and detected using a 2D heteronuclear correlation NMR experiment. This method is capable of detecting over 100 metabolites at concentrations as low as a few micromolar in biological samples, both quantitatively and reproducibly. Carboxyl-containing compounds are found in almost all metabolic pathways, and thus this new approach should find a variety of applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.