ReviewElectrophoresis2009
Electrophoresis of DNA in agarose gels, polyacrylamide gels and in free solution.
Review in Electrophoresis, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
42 citing papers in PubMed, 194 citations in OpenAlex.
- Generation of Human Bioartificial Tissues Using Agarose-Derived Biomaterials.Materials (Basel, Switzerland) · 2026Review
- Directional bone matrix mineralization in the CAM assay is governed by vascular integration and matrix remodeling.bioRxiv : the preprint server for biology · 2026Article
- Arbovirus Detection inPathogens (Basel, Switzerland) · 2025Article
- Controlled Growth of Highly Defected Zirconium-Metal-Organic Frameworks via a Reaction-Diffusion System for Water Remediation.ACS applied materials & interfaces · 2025Review
- A portable and cost-effective system for electronic nucleic acid mass measurement.Scientific reports · 2025Article
- Analysis of Inositol Phosphates via Polyacrylamide Gel Electrophoresis (PAGE).Methods in molecular biology (Clifton, N.J.) · 2025Article
- Activation Energy of SDS-Protein Complexes in Capillary Electrophoresis with Tetrahydroxyborate Cross-Linked Agarose Gels.Gels (Basel, Switzerland) · 2024Article
- Quantitative Effects of the Loop Region on Topology, Thermodynamics, and Cation Binding of DNA G-quadruplexes.ACS omega · 2024Article
- Electrophoretic Mobility Assay to Separate Supercoiled, Catenated, and Knotted DNA Molecules.Bio-protocol · 2024Article
- Anionic extracellular polymeric substances extracted from seawater-adapted aerobic granular sludge.Applied microbiology and biotechnology · 2024Article
- DNA supercoiling-induced shapes alter minicircle hydrodynamic properties.Nucleic acids research · 2023Article
- DNA supercoiling-induced shapes alter minicircle hydrodynamic properties.bioRxiv : the preprint server for biology · 2023Article
- pHAPE: a plasmid for production of DNA size marker ladders for gel electrophoresis.Biology methods & protocols · 2023Article
- A quest for cytocompatible metal organic frameworks in non-viral gene therapy: Relevance of zeolitic imidazolate framework-8.Biomaterials and biosystems · 2022Article
- Gold-Nanoparticle-Enhanced Radio-Fluorogenic Hydrogel Sensor for Low Radiation Doses in Clinical Radiotherapy.Polymers · 2022Article
- A PCR-RFLP Technique to Assess the Geographic Origin ofTropical medicine and infectious disease · 2022Article
- A handheld-type total integrated capillary electrophoresis system for SARS-CoV-2 diagnostics: Power, fluorescence detection, and data analysis by smartphone.Biosensors & bioelectronics · 2022Article
- Engineering a cell-penetrating hyperstable antibody scFv(Ras) - An extraordinary approach to cancer therapeutics.Synthetic and systems biotechnology · 2021Article
- Epitachophoresis is a novel versatile total nucleic acid extraction method.Scientific reports · 2021Article
- Two-Dimensional Gel Electrophoresis to Study the Activity of Type IIA Topoisomerases on Plasmid Replication Intermediates.Biology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
This review describes the electrophoresis of curved and normal DNA molecules in agarose gels, polyacrylamide gels and in free solution. These studies were undertaken to clarify why curved DNA molecules migrate anomalously slowly in polyacrylamide gels but not in agarose gels. Two milestone papers are cited, in which Ferguson plots were used to estimate the effective pore size of agarose and polyacrylamide gels. Subsequent studies on the effect of the electric field on agarose and polyacrylamide gel matrices, DNA interactions with the two gel matrices, and the effect of curvature on the free solution mobility of DNA are also described. The combined results suggest that the anomalously slow mobilities observed for curved DNA molecules in polyacrylamide gels are primarily due to preferential interactions of curved DNAs with the polyacrylamide gel matrix; the restrictive pore size of the matrix is of lesser importance. In free solution, DNA mobilities increase with increasing molecular mass until leveling off at a plateau value of (3.17 +/- 0.01) x 10(-4) cm2/V s in 40 mM Tris-acetate-EDTA buffer at 20 degrees C. Curved DNA molecules migrate anomalously slowly in free solution as well as in polyacrylamide gels, explaining why the Ferguson plots of curved and normal DNAs containing the same number of base pairs extrapolate to different mobilities at zero gel concentration.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.