Evidence map›Paper›PMID 19517445›Full record

ArticleElectrophoresis2009

MCE-electrochemical detection for following interactions of ssDNA and dsDNA with methylene blue.

Mario Castaño-Alvarez, Ana Fernández-la-Villa, María Teresa Fernández-Abedul, Agustín Costa-García

Open access · greenAbstract read
In one paragraph

Article in Electrophoresis, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.4field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 16 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Mario Castaño-AlvarezDepartamento de Química Física y Analítica, Universidad de Oviedo, Asturias, Spain.
Ana Fernández-la-Villa
María Teresa Fernández-Abedul
Agustín Costa-García
Universidad de Oviedo · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The interaction between the organic dye, methylene blue and DNA has been studied by MCE with electrochemical detection. Interaction produces two different signals, one corresponding to free methylene blue and other, for the complex methylene blue-DNA. The hybridization between a ssDNA and a complementary sequence, specific to the severe acute respiratory syndrome virus, has been performed and studied in a thermoplastic olefin polymer of amorphous structure CE-microchip with an end-channel gold wire detector. Moreover, studies with a longer dsDNA, an expression vector involved in the transitory or stable expression in mammals cells, pFLAG-CMV4, has also been performed.

Indexed as

DNADNA, Single-StrandedElectrochemical TechniquesElectrophoresis, CapillaryElectrophoresis, MicrochipGoldIntercalating AgentsMethylene BlueNucleic Acid HybridizationDNADNA, Single-StrandedGoldIntercalating AgentsMethylene Blue

Identifiers

PMID19517445
PMCPMC7163665
OpenAlexW1593429453

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.