ArticleGene therapy2009
Non-cell-autonomous effects of vector-expressed regulatory RNAs in mammalian heart cells.
Article in Gene therapy, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 56 citations in OpenAlex.
- Controllable gap junctions by vitamin BProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Plant and animal small RNA communications between cells and organisms.Nature reviews. Molecular cell biology · 2022Review
- Src Regulation of Cx43 Phosphorylation and Gap Junction Turnover.Biomolecules · 2020Article
- Exchange of genetic material: a new paradigm in bone cell communications.Cellular and molecular life sciences : CMLS · 2018Review
- Conflicting Roles of Connexin43 in Tumor Invasion and Growth in the Central Nervous System.International journal of molecular sciences · 2018Review
- Collateral Damage Intended-Cancer-Associated Fibroblasts and Vasculature Are Potential Targets in Cancer Therapy.International journal of molecular sciences · 2017Review
- The Role of Gap Junction-Mediated Endothelial Cell-Cell Interaction in the Crosstalk between Inflammation and Blood Coagulation.International journal of molecular sciences · 2017Review
- Uptake and impact of natural diet-derived small RNA in invertebrates: Implications for ecology and agriculture.RNA biology · 2017Review
- Formidable challenges to the notion of biologically important roles for dietary small RNAs in ingesting mammals.Genes & nutrition · 2017Review
- Hypothesis of KFrontiers in molecular neuroscience · 2017Review
- Intercellular transfer of small RNAs from astrocytes to lung tumor cells induces resistance to chemotherapy.Oncotarget · 2016Article
- Kinase programs spatiotemporally regulate gap junction assembly and disassembly: Effects on wound repair.Seminars in cell & developmental biology · 2016Review
- Gap junction mediated miRNA intercellular transfer and gene regulation: A novel mechanism for intercellular genetic communication.Scientific reports · 2016Article
- The role of neural connexins in HeLa cell mobility and intercellular communication through tunneling tubes.BMC cell biology · 2016Article
- Extracellular vesicles as carriers of microRNA, proteins and lipids in tumor microenvironment.International journal of cancer · 2016Review
- Connexin26 gap junction mediates miRNA intercellular genetic communication in the cochlea and is required for inner ear development.Scientific reports · 2015Article
- Movement of regulatory RNA between animal cells.Genesis (New York, N.Y. : 2000) · 2015Review
- Article
- Connexin43 Mediated Delivery of ADAMTS5 Targeting siRNAs from Mesenchymal Stem Cells to Synovial Fibroblasts.PloS one · 2015Article
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In mammalian cells, small regulatory RNA molecules are able to modulate gene expression in a cell-autonomous manner. In contrast, this mechanism of gene regulation can occur systemically in plants and nematodes. The existence of similar cell-to-cell transmission in mammalian cells has been explored, but generalizibilty and mechanistic insights have remained elusive. Here, we show that small regulatory RNA molecules are capable of a non-cell-autonomous effect between primary cardiac myocytes through a gap-junction-dependent mechanism. Co-culture experiments showed that both Dicer-processed small-interfering RNAs (siRNAs) and Drosha-processed microRNAs (miRNAs) were capable of target gene knockdown and physiological effects in a non-cell-autonomous manner. Target gene siRNA molecules were detected in recipient cells, indicating transfer of the primary effector molecule. All of these effects were abrogated by dominant-negative molecular suppression of gap junction function. Our results show that both siRNAs and miRNAs are capable of a non-cell-autonomous effect between mammalian cells through gap junctions. The recognition of this biological process raises the novel therapeutic prospect of a bystander effect after gene transfer to tissues bearing gap junctions and for cell engineering with a view to creating regulatory RNA donor cells that exert their influence throughout a syncytium.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.