ArticleProceedings of the National Academy of Sciences of the United States of America2009
Elimination of antiviral defense by viral RNase III.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
57 citing papers in PubMed, 1 synthesis or guideline pooled it, 153 citations in OpenAlex.
- First systematic review of the last 30 years of research on sweetpotato: elucidating the frontiers and hotspots.Frontiers in plant science · 2024Pooled it
- Pathogens That Rewrite the Rules: Ascoviruses, Elegant Manipulators of Cell Death Pathways and Architects of the Extracellular Viral Paradigm.Pathogens (Basel, Switzerland) · 2025Review
- A group of segmented viruses contains genome segments sharing homology with multiple viral taxa.Journal of virology · 2025Article
- Key Sweet Potato Viruses in Fujian Province and Their Distribution, Harmfulness, and Implications in China.Current issues in molecular biology · 2025Article
- Citrus tristeza virus p20 suppresses antiviral RNA silencing by co-opting autophagy-related protein 8 to mediate the autophagic degradation of SGS3.PLoS pathogens · 2025Article
- High-throughput characterization and phenotyping of resistance and tolerance to virus infection in sweetpotato.Virus research · 2024Article
- Analysis of Virus-Induced Double-Stranded RNA in Living Plant Cells by the dRBFC Assay.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Application of Grafting Method in Resistance Identification of Sweet Potato Virus Disease and Resistance Evaluation of Elite Sweet Potato [Plants (Basel, Switzerland) · 2023Article
- RNAi-Based Antiviral Innate Immunity in Plants.Viruses · 2022Review
- Targeting of SPCSV-RNase3 via CRISPR-Cas13 confers resistance against sweet potato virus disease.Molecular plant pathology · 2022Article
- Characterization of cassava ORANGE proteins and their capability to increase provitamin A carotenoids accumulation.PloS one · 2022Article
- Roles of small RNAs in crop disease resistance.Stress biology · 2021Review
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- Transcriptome analysis provides insights into the responses of sweet potato to sweet potato virus disease (SPVD).Virus research · 2021Article
- Synergistic Effects of aFrontiers in plant science · 2021Article
- A Glimpse of "Dicer Biology" Through the Structural and Functional Perspective.Frontiers in molecular biosciences · 2021Review
- p24Viruses · 2020Article
- Characterization of virus species associated with sweetpotato virus diseases in Burkina Faso.Plant pathology · 2020Article
- Development of FRET-based high-throughput screening for viral RNase III inhibitors.Molecular plant pathology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sweet potato (Ipomoea batatas) is an important subsistence and famine reserve crop grown in developing countries where Sweet potato chlorotic stunt virus (SPCSV; Closteroviridae), a single-stranded RNA (ssRNA) crinivirus, synergizes unrelated viruses in co-infected sweet potato plants. The most severe disease and yield losses are caused by co-infection with SPCSV and a potyvirus, Sweet potato feathery mottle virus (SPFMV; Potyviridae). Potyviruses synergize unrelated viruses by suppression of RNA silencing with the P1/HC-Pro polyprotein; however, the SPCSV-SPFMV synergism is unusual in that the potyvirus is the beneficiary. Our data show that transformation of an SPFMV-resistant sweet potato variety with the double-stranded RNA (dsRNA)-specific class 1 RNA endoribonuclease III (RNase3) of SPCSV broke down resistance to SPFMV, leading to high accumulation of SPFMV antigen and severe disease symptoms similar to the synergism in plants co-infected with SPCSV and SPFMV. RNase3-transgenic sweet potatoes also accumulated higher concentrations of 2 other unrelated viruses and developed more severe symptoms than non-transgenic plants. In leaves, RNase3 suppressed ssRNA-induced gene silencing (RNAi) in an endonuclease activity-dependent manner. It cleaved synthetic double-stranded small interfering RNAs (siRNAs) of 21, 22, and 24 bp in vitro to products of approximately 14 bp that are inactive in RNAi. It also affected total siRNA isolated from SPFMV-infected sweet potato plants, suggesting a viral mechanism for suppression of RNAi by cleavage of siRNA. Results implicate RNase3 in suppression of antiviral defense in sweet potato plants and reveal RNase3 as a protein that mediates viral synergism with several unrelated viruses, a function previously described only for P1/HC-Pro.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.