ArticlePloS one2009
Functional analysis of the VirSR phosphorelay from Clostridium perfringens.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- The presence of differentiated C2C12 muscle cells enhances toxin production and growth byVirulence · 2024Article
- The RgaS-RgaR two-component system promotes Clostridioides difficile sporulation through a small RNA and the Agr1 system.PLoS genetics · 2023Article
- The RgaS-RgaR two-component system promotesbioRxiv : the preprint server for biology · 2023Article
- Pathogenicity and virulence ofVirulence · 2021Article
- Identifying the Basis for VirS/VirR Two-Component Regulatory System Control of Clostridium perfringens Beta-Toxin Production.Journal of bacteriology · 2021Article
- A Rapid and Simple Assay Correlates In Vitro NetB Activity withMicroorganisms · 2021Article
- Interplay between Regulatory RNAs and Signal Transduction Systems during Bacterial Infection.Genes · 2020Review
- Article
- Role of RNase Y in Clostridium perfringens mRNA Decay and Processing.Journal of bacteriology · 2017Article
- Cas9 Nickase-Assisted RNA Repression Enables Stable and Efficient Manipulation of Essential Metabolic Genes inFrontiers in microbiology · 2017Article
- Imidazole as a Small Molecule Analogue in Two-Component Signal Transduction.Biochemistry · 2015Article
- Review
- Toxin plasmids of Clostridium perfringens.Microbiology and molecular biology reviews : MMBR · 2013Review
- Two-component systems are involved in the regulation of botulinum neurotoxin synthesis in Clostridium botulinum type A strain Hall.PloS one · 2012Article
- Genome sequencing and analysis of a type A Clostridium perfringens isolate from a case of bovine clostridial abomasitis.PloS one · 2012Article
- Regulation of virulence by the RevR response regulator in Clostridium perfringens.Infection and immunity · 2011Article
- Development and application of a method for counterselectable in-frame deletion in Clostridium perfringens.Applied and environmental microbiology · 2011Article
- The VirSR two-component signal transduction system regulates NetB toxin production in Clostridium perfringens.Infection and immunity · 2010Article
- Comparative genomics of VirR regulons in Clostridium perfringens strains.BMC microbiology · 2010Article
- Construction of a nontoxigenic Clostridium botulinum strain for food challenge studies.Applied and environmental microbiology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Toxin production in Clostridium perfringens is controlled by the VirSR two-component signal transduction system, which comprises the VirS sensor histidine kinase and the VirR response regulator. Other studies have concentrated on the elucidation of the genes controlled by this network; there is little information regarding the phosphorelay cascade that is the hallmark of such regulatory systems. In this study, we have examined each step in this cascade, beginning with autophosphorylation of VirS, followed by phosphotransfer from VirS to VirR. We also have studied the effects of gene dosage and phosphorylation in vivo. We have used random and site-directed mutagenesis to identify residues in VirS that are important for its function and have identified a region in the putative sensory domain of VirS that appeared to be essential for function. In vitro phosphorylation studies showed that VirSc, a truncated VirS protein that lacked the N-terminal sensory domain, was capable of autophosphorylation and could subsequently act as a phosphodonor for its cognate response regulator, VirR. Conserved residues of both VirS and VirR, including the D57 residue of VirR, were shown to be essential for this process. By use of Targetron technology, we were able to introduce a single copy of virR or virR(D57N) onto the chromosome of a virR mutant of C. perfringens. The results showed that in vivo, when virR was present in single copy, the production of wild-type levels of perfringolysin O was dependent on the presence of virS and an unaltered D57 residue in VirR. These results provide good evidence that phosphorylation is critical for VirR function.
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