ArticlePhysiological genomics2009
Skeletal muscle gene expression after myostatin knockout in mature mice.
Article in Physiological genomics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 57 citations in OpenAlex.
- The Role of Branched Chain Ketoacid Dehydrogenase Kinase (BCKDK) in Skeletal Muscle Biology and Pathogenesis.International journal of molecular sciences · 2024Article
- Mechanisms of mechanical overload-induced skeletal muscle hypertrophy: current understanding and future directions.Physiological reviews · 2023Review
- Muscle Wasting and Sarcopenia in Heart Failure-The Current State of Science.International journal of molecular sciences · 2020Review
- EffectiveInternational journal of molecular sciences · 2020Article
- Expression Levels of Long Non-Coding RNAs Change in Models of Altered Muscle Activity and Muscle Mass.International journal of molecular sciences · 2020Article
- Article
- Methyltransferase-like 21e inhibits 26S proteasome activity to facilitate hypertrophy of type IIb myofibers.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Systemic Blockade of ACVR2B Ligands Protects Myocardium from Acute Ischemia-Reperfusion Injury.Molecular therapy : the journal of the American Society of Gene Therapy · 2019Article
- Article
- Isozygous and selectable marker-free MSTN knockout cloned pigs generated by the combined use of CRISPR/Cas9 and Cre/LoxP.Scientific reports · 2016Article
- Myostatin blockade with a fully human monoclonal antibody induces muscle hypertrophy and reverses muscle atrophy in young and aged mice.Skeletal muscle · 2015Article
- Pharmacology of manipulating lean body mass.Clinical and experimental pharmacology & physiology · 2015Review
- Article
- Striking differences between knockout and wild-type mice in global gene expression variability.PloS one · 2014Article
- Nuclear entrapment and extracellular depletion of PCOLCE is associated with muscle degeneration in oculopharyngeal muscular dystrophy.BMC neurology · 2013Article
- Article
- Muscle wasting and the temporal gene expression pattern in a novel rat intensive care unit model.BMC genomics · 2011Article
- Effect of postdevelopmental myostatin depletion on myofibrillar protein metabolism.American journal of physiology. Endocrinology and metabolism · 2011Article
- Myostatin from the heart: local and systemic actions in cardiac failure and muscle wasting.American journal of physiology. Heart and circulatory physiology · 2011Review
- Gene expression profiling of skeletal muscles treated with a soluble activin type IIB receptor.Physiological genomics · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
There is much interest in developing anti-myostatin agents to reverse or prevent muscle atrophy in adults, so it is important to characterize the effects of reducing myostatin activity after normal muscle development. For assessment of the effect of loss of myostatin signaling on gene expression in muscle, RNA from mice with postdevelopmental myostatin knockout was analyzed with oligonucleotide microarrays. Myostatin was undetectable in muscle within 2 wk after Cre recombinase activation in 4-month-old male mice with floxed myostatin genes. Three months after myostatin depletion, muscle mass had increased 26% (vs. 2% after induction of Cre activity in mice with normal myostatin genes), at which time the expression of several hundred genes differed in knockout and control mice at nominal P < 0.01. In contrast to previously reported effects of constitutive myostatin knockout, postdevelopmental knockout did not downregulate expression of genes encoding slow isoforms of contractile proteins or genes encoding proteins involved in energy metabolism. Several collagen genes were expressed at 20-50% lower levels in the myostatin-deficient muscles, which had approximately 25% less collagen than normal muscles as reflected by hydroxyproline content. Most of the other genes affected by myostatin depletion have not been previously linked to myostatin signaling. Gene set enrichment analysis suggested that Smads are not the only transcription factors with reduced activity after myostatin depletion. These data reinforce other evidence that myostatin regulates collagen production in muscle and demonstrate that many of the previously reported effects of constitutive myostatin deficiency do not occur when myostatin is knocked out in mature muscles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.