ArticleMolecular & cellular proteomics : MCP2009
Proteomics analysis of Lactobacillus casei Zhang, a new probiotic bacterium isolated from traditional home-made koumiss in Inner Mongolia of China.
Article in Molecular & cellular proteomics : MCP, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Overview of Dairy-based Products with Probiotics: Fermented or Non-fermented Milk Drink.Food science of animal resources · 2024Review
- Profiling of koumiss microbiota and organic acids and their effects on koumiss taste.BMC microbiology · 2020Article
- Review
- Role ofFrontiers in microbiology · 2019Article
- A metabolomics and proteomics study of the Lactobacillus plantarum in the grass carp fermentation.BMC microbiology · 2018Article
- Cow-to-mouse fecal transplantations suggest intestinal microbiome as one cause of mastitis.Microbiome · 2018Article
- Proteomic studies on lactic acid bacteria: A review.Biochemistry and biophysics reports · 2018Review
- Using Raman spectroscopy and chemometrics to identify the growth phase of Lactobacillus casei Zhang during batch culture at the single-cell level.Microbial cell factories · 2017Article
- Adaptation ofFrontiers in microbiology · 2017Article
- Isolation and Analysis of Salt Response ofIndian journal of microbiology · 2016Article
- Isolation and Identification of Lactic Acid Bacteria from Traditional Dairy Products in Baotou and Bayannur of Midwestern Inner Mongolia and q-PCR Analysis of Predominant Species.Korean journal for food science of animal resources · 2016Article
- Identification of Oxygen-Responsive Transcripts in the Silage Inoculant Lactobacillus buchneri CD034 by RNA Sequencing.PloS one · 2015Article
- Physiological and proteomic analysis of Lactobacillus casei in response to acid adaptation.Journal of industrial microbiology & biotechnology · 2014Article
- A partial proteome reference map of the wine lactic acid bacterium Oenococcus oeni ATCC BAA-1163.Open biology · 2014Article
- Homologous overexpression of RfaH in E. coli K4 improves the production of chondroitin-like capsular polysaccharide.Microbial cell factories · 2013Article
- Transcriptome analysis of probiotic Lactobacillus casei Zhang during fermentation in soymilk.Journal of industrial microbiology & biotechnology · 2012Article
- In vitro antagonistic activity of Lactobacillus casei against Helicobacter pylori.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
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9 authors.
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Abstract
Lactobacillus casei Zhang, isolated from traditional home-made koumiss in Inner Mongolia of China, was considered as a new probiotic bacterium by probiotic selection tests. We carried out a proteomics study to identify and characterize proteins expressed by L. casei Zhang in the exponential phase and stationary phase. Cytosolic proteins of the strain cultivated in de Man, Rogosa, and Sharpe broth were resolved by two-dimensional gel electrophoresis using pH 4-7 linear gradients. The number of protein spots quantified from the gels was 487 +/- 21 (exponential phase) and 494 +/- 13 (stationary phase) among which a total of 131 spots were identified by MALDI-TOF/MS and/or MALDI-TOF/TOF according to significant growth phase-related differences or high expression intensity proteins. Accompanied by the cluster of orthologous groups (COG), codon adaptation index (CAI), and GRAVY value analysis, the study provided a very first insight into the profile of protein expression as a reference map of L. casei. Forty-seven spots were also found in the study that showed statistically significant differences between exponential phase and stationary phase. Thirty-three of the spots increased at least 2.5-fold in the stationary phase in comparison with the exponential phase, including 19 protein spots (e.g. Hsp20, DnaK, GroEL, LuxS, pyruvate kinase, and GalU) whose intensity up-shifted above 3.0-fold. Transcriptional profiles were conducted to confirm several important differentially expressed proteins by using real time quantitative PCR. The analysis suggests that the differentially expressed proteins were mainly categorized as stress response proteins and key components of central and intermediary metabolism, indicating that these proteins might play a potential important role for the adaptation to the surroundings, especially the accumulation of lactic acid in the course of growth, and the physiological processes in bacteria cell.
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