ArticleThe Biochemical journal2009
The 5' terminal uracil of let-7a is critical for the recruitment of mRNA to Argonaute2.
Article in The Biochemical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 36 citations in OpenAlex.
- Identification of conserved miRNAs and their targets in Jatropha curcas: an in silico approach.Journal, genetic engineering & biotechnology · 2023Article
- siRNA potency enhancement via chemical modifications of nucleotide bases at the 5'-end of the siRNA guide strand.RNA (New York, N.Y.) · 2021Article
- Regulation and imaging of gene expressionChemical science · 2017Article
- Autonomous Generation and Loading of DNA Guides by Bacterial Argonaute.Molecular cell · 2017Article
- Conformational Dynamics of Ago-Mediated Silencing Processes.International journal of molecular sciences · 2015Review
- Article
- Short interfering RNA guide strand modifiers from computational screening.Journal of the American Chemical Society · 2013Article
- Regulation of huntingtin gene expression by miRNA-137, -214, -148a, and their respective isomiRs.International journal of molecular sciences · 2013Article
- Article
- SNPing cancer in the bud: microRNA and microRNA-target site polymorphisms as diagnostic and prognostic biomarkers in cancer.Pharmacology & therapeutics · 2013Review
- Arabidopsis Argonaute MID domains use their nucleotide specificity loop to sort small RNAs.The EMBO journal · 2012Article
- Functional parameters of Dicer-independent microRNA biogenesis.RNA (New York, N.Y.) · 2012Article
- Human miR-1271 is a miR-96 paralog with distinct non-conserved brain expression pattern.Nucleic acids research · 2011Article
- Structural basis for 5'-nucleotide base-specific recognition of guide RNA by human AGO2.Nature · 2010Article
- Uridylation of mature miRNAs and siRNAs by the MUT68 nucleotidyltransferase promotes their degradation in Chlamydomonas.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Small RNAs modulate gene expression by forming a ribonucleoprotein complex with Argonaute proteins and directing them to specific complementary sites in target nucleic acids. However, the interactions required for the recruitment of the target nucleic acid to the ribonucleoprotein complex are poorly understood. In the present manuscript we have investigated this question by using let-7a, Argonaute2 and a fully complementary mRNA target. Importantly, we have found that recombinant Argonaute2 is sufficient to direct let-7a guided cleavage of mRNA. Thus this model system has allowed us to investigate the mechanistic basis of silencing in vitro and in vivo. Current models suggest that Argonaute proteins bind to both the 5' and 3' termini of the guide RNA. We have found that the termini of the let-7a microRNA are indeed critical, since circular let-7a does not support mRNA cleavage. However, the 5' end is the key determinant, since its deletion abrogates activity. Surprisingly, we have found that alteration of the 5' terminal uracil compromises mRNA cleavage. Importantly, we have found that substitution of this base has little effect upon the formation of the binary let-7a-Argonaute2 complex, but inhibits the formation of the ternary let-7a-Argonaute2-mRNA complex. Thus we conclude that the interaction of the 5' uracil base with Argonaute2 plays a critical and novel role in the recruitment of mRNA.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.