ArticleTransgenic research2010
Transgene silencing in grapevines transformed with GFLV resistance genes: analysis of variable expression of transgene, siRNAs production and cytosine methylation.
Article in Transgenic research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 48 citations in OpenAlex.
- Review
- Comparative Metabolomic Analysis of Four Fabaceae and Relationship to In Vitro Nematicidal Activity againstMolecules (Basel, Switzerland) · 2022Article
- A single resistance factor to solve vineyard degeneration due to grapevine fanleaf virus.Communications biology · 2021Article
- Double-Stranded RNAs (dsRNAs) as a Sustainable Tool against Gray Mold (Biomolecules · 2020Article
- In silico identification and computational characterization of endogenous small interfering RNAs from diverse grapevine tissues and stages.Genes & genomics · 2018Article
- Expression of disease resistance in genetically modified grapevines correlates with the contents of viral sequences in the T-DNA and global genome methylation.Transgenic research · 2018Article
- Nanobody-mediated resistance to Grapevine fanleaf virus in plants.Plant biotechnology journal · 2018Article
- Delivery of Hairpin RNAs and Small RNAs Into Woody and Herbaceous Plants by Trunk Injection and Petiole Absorption.Frontiers in plant science · 2018Article
- Citrus psorosis virus coat protein-derived hairpin construct confers stable transgenic resistance in citrus against psorosis A and B syndromes.Transgenic research · 2017Article
- Grafting: A Technique to Modify Ion Accumulation in Horticultural Crops.Frontiers in plant science · 2016Review
- Levels of DNA methylation and transcript accumulation in leaves of transgenic maize varieties.Environmental sciences Europe · 2016Article
- Unravelling rootstock×scion interactions to improve food security.Journal of experimental botany · 2015Review
- Epigenetic silencing in transgenic plants.Frontiers in plant science · 2015Review
- Biosafety considerations of RNAi-mediated virus resistance in fruit-tree cultivars and in rootstock.Transgenic research · 2013Review
- Progressive 35S promoter methylation increases rapidly during vegetative development in transgenic Nicotiana attenuata plants.BMC plant biology · 2013Article
- Genetic transformation of fruit trees: current status and remaining challenges.Transgenic research · 2012Review
- Transient expression of artificial microRNAs targeting Grapevine fanleaf virus and evidence for RNA silencing in grapevine somatic embryos.Transgenic research · 2012Article
- The grapevine root-specific aquaporin VvPIP2;4N controls root hydraulic conductance and leaf gas exchange under well-watered conditions but not under water stress.Plant physiology · 2012Article
- Agrobacterium-mediated genetic transformation of Coffea arabica (L.) is greatly enhanced by using established embryogenic callus cultures.BMC plant biology · 2011Article
- Paramutation of tobacco transgenes by small RNA-mediated transcriptional gene silencing.Epigenetics · 2011Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Eight transgenic grapevine lines transformed with the coat protein gene of Grapevine fanleaf virus (GFLV-CP) were analyzed for a correlation between transgene expression, siRNAs production and DNA methylation. Bisulphite genome sequencing was used for a comprehensive analysis of DNA methylation. Methylated cytosine residues of CpG and CpNpG sites were detected in the GFLV-CP transgene, in the T7 terminator and in the 35S promoter of three grapevines without transgene expression, but no detectable level of siRNAs was recorded in these lines. The detailed analysis of 8 lines revealed the complex arrangements of T-DNA and integrated binary vector sequences as crucial factors that influence transgene expression. After inoculation with GFLV, no change in the levels of cytosine methylation was observed, but transgenic and untransformed plants produced short siRNAs (21-22 nt) indicating that the grapevine plants responded to GFLV infection by activating a post-transcriptional gene silencing mechanism.
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