ArticlePhysical chemistry chemical physics : PCCP2009
Using electrical and optical tweezers to facilitate studies of molecular motors.
Article in Physical chemistry chemical physics : PCCP, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- Myosin-5 varies its step length to carry cargo straight along the irregular F-actin track.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Membrane-bound myosin IC drives the chiral rotation of the gliding actin filament around its longitudinal axis.Scientific reports · 2023Article
- Electromagnetic Forces and Torques: From Dielectrophoresis to Optical Tweezers.Chemical reviews · 2023Review
- Hot embossed polyethylene through-hole chips for bead-based microfluidic devices.Biosensors & bioelectronics · 2013Article
- A branched kinetic scheme describes the mechanochemical coupling of Myosin Va processivity in response to substrate.Biophysical journal · 2012Article
- Lever-arm mechanics of processive myosins.Biophysical journal · 2011Review
- Comparison of Brownian-dynamics-based estimates of polymer tension with direct force measurements.Physical review. E, Statistical, nonlinear, and soft matter physics · 2010Article
- Single-molecule stepping and structural dynamics of myosin X.Nature structural & molecular biology · 2010Article
- Twirling motion of actin filaments in gliding assays with nonprocessive Myosin motors.Biophysical journal · 2009Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Dielectrophoresis was used to stretch and suspend actin filaments across a trench etched between two electrodes patterned on a glass slide. Optical tweezers were used to bring a motor protein-coated bead into close proximity to a pre-selected, suspended actin filament, facilitating the attachment of the myosin-coated bead to the filament. The clearance beneath the filament allowed the bead to move freely along and around its filamentous track, unhindered by solid surfaces. Using defocused images, the three-dimensional position of the bead was tracked as a function of time to obtain its trajectory. Experiments were carried out with myosin V and myosin X. Both motor proteins followed left-handed helical paths with the myosin X motor exhibiting a shorter pitch than the myosin V. The combined use of electrostatic and optical tweezers facilitates the preparation of motility assays with suspended tracks. Variants of this technique will enable higher complexity experiments in vitro to better understand the behavior of motors in cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.