ArticleNucleic acids research2009
The incorporation of the novel histone variant H2AL2 confers unusual structural and functional properties of the nucleosome.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 46 citations in OpenAlex.
- Higher-order genome architecture of spermatids guides chromatin assembly in mature sperm.Research square · 2026Article
- Distinct functions in fertility and patterns of paternal incorporation of the histone H2A variants HTAS-1 and HTZ-1 in C. elegans.Genetics · 2026Article
- Roles of Histone H2A Variants in Cancer Development, Prognosis, and Treatment.International journal of molecular sciences · 2024Review
- Casting histone variants during mammalian reproduction.Chromosoma · 2023Review
- Emerging evidence that the mammalian sperm epigenome serves as a template for embryo development.Nature communications · 2023Review
- Unusual nucleosome formation and transcriptome influence by the histone H3mm18 variant.Nucleic acids research · 2022Article
- The Art of Packaging the Sperm Genome: Molecular and Structural Basis of the Histone-To-Protamine Exchange.Frontiers in endocrinology · 2022Review
- Exposure to drugs of abuse induce effects that persist across generations.International review of neurobiology · 2021Article
- Biparental contributions of the H2A.B histone variant control embryonic development in mice.PLoS biology · 2020Article
- Review
- The roles of histone variants in fine-tuning chromatin organization and function.Nature reviews. Molecular cell biology · 2020Review
- Review
- Article
- Generation of Remosomes by the SWI/SNF Chromatin Remodeler Family.Scientific reports · 2019Article
- DNA accessibility of chromatosomes quantified by automated image analysis of AFM data.Scientific reports · 2019Article
- Histone variants: critical determinants in tumour heterogeneity.Frontiers of medicine · 2019Review
- Evolutionary origins and diversification of testis-specific short histone H2A variants in mammals.Genome research · 2018Article
- Structure and Dynamics of a 197 bp Nucleosome in Complex with Linker Histone H1.Molecular cell · 2017Article
- Linker Histone in Diseases.International journal of biological sciences · 2017Review
- MS_HistoneDB, a manually curated resource for proteomic analysis of human and mouse histones.Epigenetics & chromatin · 2017Article
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Authors and funding
9 authors at 7 institutions in 1 country.
Funding
Abstract
In this work we have studied the properties of the novel mouse histone variant H2AL2. H2AL2 was used to reconstitute nucleosomes and the structural and functional properties of these particles were studied by a combination of biochemical approaches, atomic force microscopy (AFM) and electron cryo-microscopy. DNase I and hydroxyl radical footprinting as well as micrococcal and exonuclease III digestion demonstrated an altered structure of the H2AL2 nucleosomes all over the nucleosomal DNA length. Restriction nuclease accessibility experiments revealed that the interactions of the H2AL2 histone octamer with the ends of the nucleosomal DNA are highly perturbed. AFM imaging showed that the H2AL2 histone octamer was complexed with only approximately 130 bp of DNA. H2AL2 reconstituted trinucleosomes exhibited a type of a 'beads on a string' structure, which was quite different from the equilateral triangle 3D organization of conventional H2A trinucleosomes. The presence of H2AL2 affected both the RSC and SWI/SNF remodeling and mobilization of the variant particles. These unusual properties of the H2AL2 nucleosomes suggest a specific role of H2AL2 during mouse spermiogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.