ArticleNucleic acids research2009
Extensive DNA mimicry by the ArdA anti-restriction protein and its role in the spread of antibiotic resistance.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
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Who cites it
81 citing papers in PubMed, 122 citations in OpenAlex.
- Characteristics of an NDM-1-producing Klebsiella pneumoniae strain belonging to ST105.Antonie van Leeuwenhoek · 2026Article
- Restriction-modification systems and prophages drive genomic diversification in Priestia megaterium.Biology direct · 2026Article
- A bacteriophage-encoded protein gp119 suppresses deoxyinosine repair to protect hypermutated viral genome.iScience · 2026Article
- A methylome-derived mbioRxiv : the preprint server for biology · 2026Article
- Optimizing phage therapy with antidefense proteins acquired from the environment.Frontiers in microbiology · 2026Article
- Biology of host-dependent restriction-modification in prokaryotes.EcoSal Plus · 2025Review
- Distinct Phage-Encoded Enzymes for Substitution of Deoxythymidine by Deoxyuridine in Phage Genomes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- A new family of small ArdA proteins reveals antirestriction activity.Journal of bacteriology · 2025Article
- Comparative and functional genomic analysis of foreign DNA defense mechanisms inMicrobiology spectrum · 2025Article
- The extended mobility of plasmids.Nucleic acids research · 2025Review
- Activation of bacterial programmed cell death by phage inhibitors of host immunity.Molecular cell · 2025Article
- Acquired Bacterial Resistance to Antibiotics and Resistance Genes: From Past to Future.Antibiotics (Basel, Switzerland) · 2025Review
- Various plasmid strategies limit the effect of bacterial restriction-modification systems against conjugation.Nucleic acids research · 2024Article
- Ocr-mediated suppression of BrxX unveils a phage counter-defense mechanism.Nucleic acids research · 2024Article
- RecA-dependent or independent recombination of plasmid DNA generates a conflict with the host EcoKI immunity by launching restriction alleviation.Nucleic acids research · 2024Article
- Accumulation of defense systems in phage-resistant strains ofScience advances · 2024Article
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- Isolation of phages infecting the abundant freshwater Actinobacteriota order 'Ca. Nanopelagicales'.The ISME journal · 2023Article
- Broadness and specificity: ArdB, ArdA, and Ocr against various restriction-modification systems.Frontiers in microbiology · 2023Article
21 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 2 institutions in 1 country.
Funding
Abstract
The ardA gene, found in many prokaryotes including important pathogenic species, allows associated mobile genetic elements to evade the ubiquitous Type I DNA restriction systems and thereby assist the spread of resistance genes in bacterial populations. As such, ardA contributes to a major healthcare problem. We have solved the structure of the ArdA protein from the conjugative transposon Tn916 and find that it has a novel extremely elongated curved cylindrical structure with defined helical grooves. The high density of aspartate and glutamate residues on the surface follow a helical pattern and the whole protein mimics a 42-base pair stretch of B-form DNA making ArdA by far the largest DNA mimic known. Each monomer of this dimeric structure comprises three alpha-beta domains, each with a different fold. These domains have the same fold as previously determined proteins possessing entirely different functions. This DNA mimicry explains how ArdA can bind and inhibit the Type I restriction enzymes and we demonstrate that 6 different ardA from pathogenic bacteria can function in Escherichia coli hosting a range of different Type I restriction systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.