ArticleBiotechnology for biofuels2009
Yield-determining factors in high-solids enzymatic hydrolysis of lignocellulose.
Article in Biotechnology for biofuels, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 99 papers.
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Who cites it
99 citing papers in PubMed, 640 citations in OpenAlex.
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- Sustainable Production of Poly(3-hydroxybutyrate) Using Eucalyptus Bark: Integration with Green Downstream Processing.ACS sustainable chemistry & engineering · 2026Article
- Valorization of barley bagasse for lactic acid production using an enzymatic cocktail produced by Aspergillus brasiliensis.Archives of microbiology · 2026Article
- Efficient 2G Ethanol Production via Optimized Dilute Acid Pretreatment, High-Solids Enzymatic Hydrolysis, and High-Temperature Fermentation.ACS omega · 2026Article
- Overcoming the Lignin Barrier in Brewer's Spent Grain: Synergistic Effect of Additives on Enzymatic Hydrolysis.ACS omega · 2025Article
- Optimal production of Phanerochaete chrysosporium manganese peroxidases and Trametes sp. C30 laccase hybrid Lac131 in Aspergillus niger for lignin bioconversion.Biotechnology for biofuels and bioproducts · 2025Article
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- Towards polycotton waste valorisation: depolymerisation of cotton to glucose with polyester preservation.RSC sustainability · 2025Review
- Efficient enzymatic hydrolysis of sweet potato residue by fed-batch method to prepare high- concentration glucose.Bioprocess and biosystems engineering · 2025Article
- Are cellulases slow? Kinetic and thermodynamic limitations for enzymatic breakdown of cellulose.BBA advances · 2025Review
- Synergistic effects of enzyme-microbe co-fermentation on the nutritional quality and digestibility of corn stalks.Frontiers in microbiology · 2025Article
- Generation and characterization of two acid-resistant macrocinComputational and structural biotechnology journal · 2024Article
- Fed-Batch Strategy Achieves the Production of High Concentration Fermentable Sugar Solution and Cellulosic Ethanol from Pretreated Corn Stover and Corn Cob.International journal of molecular sciences · 2024Article
- Enhancing enzymatic saccharification yields of cellulose at high solid loadings by combining different LPMO activities.Biotechnology for biofuels and bioproducts · 2024Article
- Article
- LPMO-supported saccharification of biomass: effects of continuous aeration of reaction mixtures with variable fractions of water-insoluble solids and cellulolytic enzymes.Biotechnology for biofuels and bioproducts · 2023Article
- Review
- Rio Tinto as a niche for acidophilus enzymes of industrial relevance.Microbial biotechnology · 2023Article
- Metaproteomics reveals enzymatic strategies deployed by anaerobic microbiomes to maintain lignocellulose deconstruction at high solids.Nature communications · 2022Article
39 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWorking at high solids (substrate) concentrations is advantageous in enzymatic conversion of lignocellulosic biomass as it increases product concentrations and plant productivity while lowering energy and water input. However, for a number of lignocellulosic substrates it has been shown that at increasing substrate concentration, the corresponding yield decreases in a fashion which can not be explained by current models and knowledge of enzyme-substrate interactions. This decrease in yield is undesirable as it offsets the advantages of working at high solids levels. The cause of the 'solids effect' has so far remained unknown.
resultsThe decreasing conversion at increasing solids concentrations was found to be a generic or intrinsic effect, describing a linear correlation from 5 to 30% initial total solids content (w/w). Insufficient mixing has previously been shown not to be involved in the effect. Hydrolysis experiments with filter paper showed that neither lignin content nor hemicellulose-derived inhibitors appear to be responsible for the decrease in yields. Product inhibition by glucose and in particular cellobiose (and ethanol in simultaneous saccharification and fermentation) at the increased concentrations at high solids loading plays a role but could not completely account for the decreasing conversion. Adsorption of cellulases was found to decrease at increasing solids concentrations. There was a strong correlation between the decreasing adsorption and conversion, indicating that the inhibition of cellulase adsorption to cellulose is causing the decrease in yield.
conclusionInhibition of enzyme adsorption by hydrolysis products appear to be the main cause of the decreasing yields at increasing substrate concentrations in the enzymatic decomposition of cellulosic biomass. In order to facilitate high conversions at high solids concentrations, understanding of the mechanisms involved in high-solids product inhibition and adsorption inhibition must be improved.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.