ArticleYeast (Chichester, England)2009
Additional cassettes for epitope and fluorescent fusion proteins in Candida albicans.
Article in Yeast (Chichester, England), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 69 citations in OpenAlex.
- A CandiChrome toolkit for multicolor labeling ofbioRxiv : the preprint server for biology · 2026Article
- Transcriptome and proteome profiling reveals complex adaptations of Candida parapsilosis cells assimilating hydroxyaromatic carbon sources.PLoS genetics · 2022Article
- Fluorescent toys 'n' tools lighting the way in fungal research.FEMS microbiology reviews · 2021Article
- Epigenetic cell fate in Candida albicans is controlled by transcription factor condensates acting at super-enhancer-like elements.Nature microbiology · 2020Article
- Overexpression ofMicroorganisms · 2019Article
- Candida parapsilosis: from Genes to the Bedside.Clinical microbiology reviews · 2019Review
- Article
- The Zebrafish as a Model Host for Invasive Fungal Infections.Journal of fungi (Basel, Switzerland) · 2018Review
- Candida albicans Cdc15 is essential for mitotic exit and cytokinesis.Scientific reports · 2018Article
- Localizing Antifungal Drugs to the Correct Organelle Can Markedly Enhance their Efficacy.Angewandte Chemie (International ed. in English) · 2018Article
- Article
- Candida albicans and Pseudomonas aeruginosa Interact To Enhance Virulence of Mucosal Infection in Transparent Zebrafish.Infection and immunity · 2017Article
- A Bimolecular Fluorescence Complementation Tool for Identification of Protein-Protein Interactions inG3 (Bethesda, Md.) · 2017Article
- Generation of Fluorescent Protein Fusions in Candida Species.Journal of visualized experiments : JoVE · 2017Article
- Identification of small molecules that disrupt vacuolar function in the pathogen Candida albicans.PloS one · 2017Article
- A review on detection methods used for foodborne pathogens.The Indian journal of medical research · 2016Review
- Neutrophil Attack Triggers Extracellular Trap-Dependent Candida Cell Wall Remodeling and Altered Immune Recognition.PLoS pathogens · 2016Article
- Opportunistic pathogen Candida albicans elicits a temporal response in primary human mast cells.Scientific reports · 2015Article
- Fungal model systems and the elucidation of pathogenicity determinants.Fungal genetics and biology : FG & B · 2014Review
- Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Epitope tags that confer specific properties, including affinity for resins or antibodies or detection by fluorescence microscopy, are highly useful for biochemical and cell biological investigations. In Candida albicans and several other related yeasts, the CUG codon specifies serine instead of leucine, requiring that molecular tools be customized for use in this important human fungal pathogen. Here we report the construction of a set of plasmids containing 13-Myc, 3HA, GST, V5 or His9 epitope cassettes that facilitate PCR-mediated construction of epitope-tagged proteins. Common primer sets amplify the different tags with two different selectable markers. In addition, we report construction of a codon-optimized Discosoma red fluorescent protein (DsRFP) gene. Like mCherryRFP, this DsRFP signal is detectable in transformants at the colony level and is useful in double-labelling experiments with green fluorescent protein (GFP). Finally, we describe a construct that directs PCR-mediated two-step insertion of GFP internal to a coding sequence, which facilitates tagging of secreted proteins, including GPI-anchor cell wall proteins that require endogenous N- and C-termini for function. These reagents expand the repertoire of molecular tools available for working with C. albicans and other members of the CUG clade of pathogenic yeasts.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.