ArticlePlant cell reports2009
Enhanced flavonoid production in hairy root cultures of Glycyrrhiza uralensis Fisch by combining the over-expression of chalcone isomerase gene with the elicitation treatment.
Article in Plant cell reports, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 146 citations in OpenAlex.
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- Glycyrrhiza, a commonly used medicinal herb: Review of species classification, pharmacology, active ingredient biosynthesis, and synthetic biology.Journal of advanced research · 2025Review
- Biofortified Calcium Phosphate Nanoparticles Elicit Secondary Metabolite Production in Carob Callus via Biosynthetic Pathway Activation.Plants (Basel, Switzerland) · 2025Article
- Transcriptome of Two-Hybrid Poplar (Biology · 2025Article
- Anthocyanin metabolites and related regulatory genes analysis in leaves of Acer Pseudosieboldianum mutant during different periods of color change.BMC genomics · 2025Article
- SWATH-MS based proteomics reveals the role of photosynthesis related proteins and secondary metabolic pathways in the colored leaves of sweet olive (Osmanthus fragrans).BMC genomics · 2024Article
- Structural and Interactional Analysis of the Flavonoid Pathway Proteins: Chalcone Synthase, Chalcone Isomerase and Chalcone Isomerase-like Protein.International journal of molecular sciences · 2024Article
- Evaluation of pathways to thePlant direct · 2022Article
- De novo transcriptome revealed genes involved in anthocyanin biosynthesis, transport, and regulation in a mutant of Acer pseudosieboldianum.BMC genomics · 2022Article
- Hairy Root Cultures as a Source of Polyphenolic Antioxidants: Flavonoids, Stilbenoids and Hydrolyzable Tannins.Plants (Basel, Switzerland) · 2022Review
- Glycyrrhiza uralensis Fisch. Root-associated microbiota: the multifaceted hubs associated with environmental factors, growth status and accumulation of secondary metabolites.Environmental microbiome · 2022Article
- Comparative Analysis of the Chalcone-Flavanone Isomerase Genes in SixFrontiers in genetics · 2022Article
- Article
- Physio-chemical responses of exogenous calcium nanoparticle and putrescine polyamine in Saffron (Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Homology modeling and molecular dynamics based insights into3 Biotech · 2020Article
- In vitro production and distribution of flavonoids inJournal of food science and technology · 2020Article
- Arbuscular Mycorrhizal Fungi Can Compensate for the Loss of Indigenous Microbial Communities to Support the Growth of Liquorice (Plants (Basel, Switzerland) · 2019Article
- Elicitation: A biotechnological tool for enhanced production of secondary metabolites in hairy root cultures.Engineering in life sciences · 2019Review
- Development of an efficient root transgenic system for pigeon pea and its application to other important economically plants.Plant biotechnology journal · 2019Article
- Molecular cloning and functional characterization of chalcone isomerase from Carthamus tinctorius.AMB Express · 2019Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Economically important compounds, such as licorice flavonoids, are present in insufficient amounts in the hairy roots. To overcome this problem, we took the transgenic approach combined with the elicitation technique to increase the flavonoid production. The Glycyrrhiza uralensis Fisch cDNA encoding chalcone isomerase gene (chi) was over-expressed in hairy roots of G. uralensis Fisch mediated by the disarmed Agrobacterium rhizogenes A4. Stable genetic transformation was confirmed by Southern blot analysis. The transgenic and wild cultures were subsequently elicited with PEG8000 (2%) alone, yeast extract (YE) (0.1%) alone, or both of them, and then the total flavonoids were extracted and measured. The results showed that over a culture period of 3 weeks, the wild-type hairy roots, the untreated transgenic hairy roots, and the double-treated transgenic hairy roots accumulated 0.842, 1.394, and 2.838 (g/100 g DW) of total flavonoids, respectively. Moreover, the enhanced accumulation of flavonoids were correlated with the elevated level of chi transcripts and CHI activity, confirming the key role of chi in the flavonoids synthesis. This research demonstrated that the combination of the metabolic engineering and PEG8000-YE elicitation treatment was an effective strategy to increase the flavonoids production in hairy roots of G. uralensis Fisch.
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