ArticleMolecular vision2009
Increased isolevuglandin-modified proteins in glaucomatous astrocytes.
Article in Molecular vision, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The trial behind it
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Who cites it
13 citing papers in PubMed.
- Protein adduction causes non-mutational inhibition of p53 tumor suppressor.Cell reports · 2023Article
- Uncoupling proteins in the mitochondrial defense against oxidative stress.Progress in retinal and eye research · 2021Review
- Isolevuglandins as mediators of disease and the development of dicarbonyl scavengers as pharmaceutical interventions.Pharmacology & therapeutics · 2020Review
- Molecular Structures of Isolevuglandin-Protein Cross-Links.Chemical research in toxicology · 2016Article
- Isolevuglandin adducts in disease.Antioxidants & redox signaling · 2015Review
- Lipid peroxidation generates biologically active phospholipids including oxidatively N-modified phospholipids.Chemistry and physics of lipids · 2014Review
- A decade of proteomics studies of glaucomatous neurodegeneration.Proteomics. Clinical applications · 2014Review
- Pretreatment with pyridoxamine mitigates isolevuglandin-associated retinal effects in mice exposed to bright light.The Journal of biological chemistry · 2013Article
- A proteomics view of the molecular mechanisms and biomarkers of glaucomatous neurodegeneration.Progress in retinal and eye research · 2013Review
- Posttranslational modification by an isolevuglandin diminishes activity of the mitochondrial cytochrome P450 27A1.Journal of lipid research · 2013Article
- Proteomics analyses of human optic nerve head astrocytes following biomechanical strain.Molecular & cellular proteomics : MCP · 2012Article
- The immune response in glaucoma: a perspective on the roles of oxidative stress.Experimental eye research · 2011Review
- An (1)O2 route to γ-hydroxyalkenal phospholipids by vitamin E-induced fragmentation of hydroperoxydiene-derived endoperoxides.Chemical research in toxicology · 2011Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
purposeLipid oxidation has been proposed to be a factor in the pathophysiology of glaucoma. We investigated whether elevated levels of isolevuglandin (iso[4]LGE(2)) protein adducts are associated with astrocytes derived from the glaucomatous optic nerve head. In addition, we examined whether the iso[4]LGE(2) protein adducts are altered following exposure of astrocytes to elevated pressure.
methodsAstrocytes were isolated from rat brain cortex and human optic nerve and were subjected to pressure treatments, western blot analyses, liquid chromatography tandem mass spectrometry, and immunocytochemistry.
resultsElevated levels of isolevuglandin (iso[4]LGE(2)) protein adducts were associated with astrocytes derived from the glaucomatous (n=10) optic nerve head when compared to those from controls (n=6). Astrocytes subjected to in vitro pressure treatment resulted in increased levels of iso[4]LGE(2) protein adducts. Pressure exposure and the recovery period affect iso[4]LGE(2) protein modification, and pyridoxamine was effective in decreasing the appearance of iso[4]LGE(2) protein adduct immunoreactivity when applied immediately after pressure treatment.
conclusionsThese results suggest that the elevated iso[4]LGE(2) protein adduct immunoreactivity observed in glaucomatous astrocytes may be due to chronic and/or prolonged exposure to pressure, and pyridoxamine may have prophylactic utility against such oxidative protein modification.
Indexed as
Identifiers
19503745PMC2690965What OpenQuestion holds
Registered trials
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