ArticleApplied and environmental microbiology2009
Structure-based rational design of a phosphotriesterase.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 42 citations in OpenAlex.
- Applications of Microbial Organophosphate-Degrading Enzymes to Detoxification of Organophosphorous Compounds for Medical Countermeasures against Poisoning and Environmental Remediation.International journal of molecular sciences · 2024Review
- Coupling Peptide-Based Encapsulation of Enzymes with Bacteria for Paraoxon Bioremediation.ACS applied materials & interfaces · 2024Article
- The impact of molecular variants, crystallization conditions and the space group on ligand-protein complexes: a case study on bacterial phosphotriesterase.Acta crystallographica. Section D, Structural biology · 2023Article
- Overview of a bioremediation tool: organophosphorus hydrolase and its significant application in the food, environmental, and therapy fields.Applied microbiology and biotechnology · 2021Review
- Pollen-inspired enzymatic microparticles to reduce organophosphate toxicity in managed pollinators.Nature food · 2021Article
- Efficacy of an organophosphorus hydrolase enzyme (OpdA) in human serum and minipig models of organophosphorus insecticide poisoning.Clinical toxicology (Philadelphia, Pa.) · 2020Article
- 300-Fold increase in production of the Zn2+-dependent dechlorinase TrzN in soluble form via apoenzyme stabilization.Applied and environmental microbiology · 2014Article
- Use of OpdA, an organophosphorus (OP) hydrolase, prevents lethality in an African green monkey model of acute OP poisoning.Toxicology · 2014Article
- A 5000-fold increase in the specificity of a bacterial phosphotriesterase for malathion through combinatorial active site mutagenesis.PloS one · 2014Article
- From protein engineering to immobilization: promising strategies for the upgrade of industrial enzymes.International journal of molecular sciences · 2013Review
- Mining electron density for functionally relevant protein polysterism in crystal structures.Cellular and molecular life sciences : CMLS · 2011Review
- The evolution of new enzyme function: lessons from xenobiotic metabolizing bacteria versus insecticide-resistant insects.Evolutionary applications · 2011Article
- Pharmacokinetics of OpdA, an organophosphorus hydrolase, in the African green monkey.Biochemical pharmacology · 2010Article
- Kinetics and efficacy of an organophosphorus hydrolase in a rodent model of methyl-parathion poisoning.Academic emergency medicine : official journal of the Society for Academic Emergency Medicine · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In silico substrate docking of both stereoisomers of the pesticide chlorfenvinphos (CVP) in the phosphotriesterase from Agrobacterium radiobacter identified two residues (F131 and W132) that prevent productive substrate binding and cause stereospecificity. A variant (W131H/F132A) was designed that exhibited ca. 480-fold and 8-fold increases in the rate of Z-CVP and E-CVP hydrolysis, respectively, eliminating stereospecificity.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.