ArticleProceedings of the National Academy of Sciences of the United States of America2009
An externally tunable bacterial band-pass filter.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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Who cites it
71 citing papers in PubMed.
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- High-Resolution Patterned Delivery of Chemical Signals From 3D-Printed Picoliter Droplet Networks.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Concentration-Dependent CsrA Regulation of theACS synthetic biology · 2025Article
- Strong segregation promotes self-destructive cooperation.The ISME journal · 2025Article
- Functional predictability of universal gene circuits in diverse microbial hosts.Quantitative biology (Beijing, China) · 2024Article
- Protein-based bandpass filters for controlling cellular signaling with chemical inputs.Nature chemical biology · 2024Article
- Article
- Genes Vary Greatly in Their Propensity for Collateral Fitness Effects of Mutations.Molecular biology and evolution · 2023Article
- Generation of Realistic Gene Regulatory Networks by Enriching for Feed-Forward Loops.Frontiers in genetics · 2022Article
- Biological signal processing filters via engineering allosteric transcription factors.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Generation of extracellular morphogen gradients: the case for diffusion.Nature reviews. Genetics · 2021Review
- Low-N protein engineering with data-efficient deep learning.Nature methods · 2021Article
- Exploiting natural chemical photosensitivity of anhydrotetracycline and tetracycline for dynamic and setpoint chemo-optogenetic control.Nature communications · 2020Article
- Antibiotic Degradation by Commensal Microbes Shields Pathogens.Infection and immunity · 2020Article
- Cell-in-the-loop pattern formation with optogenetically emulated cell-to-cell signaling.Nature communications · 2020Article
- Automated Design of Pluripotent Stem Cell Self-Organization.Cell systems · 2019Article
- Fitness Effects of Single Amino Acid Insertions and Deletions in TEM-1 β-Lactamase.Journal of molecular biology · 2019Article
- Pervasive Pairwise Intragenic Epistasis among Sequential Mutations in TEM-1 β-Lactamase.Journal of molecular biology · 2019Article
- Selection of Protein-Protein Interactions of Desired Affinities with a Bandpass Circuit.Journal of molecular biology · 2019Article
- Converting a Periplasmic Binding Protein into a Synthetic Biosensing Switch through Domain Insertion.BioMed research international · 2019Review
11 more citing papers are in PubMed but not listed here.
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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The current paradigm for tuning synthetic biological systems is through re-engineering system components. Biological systems designed with the inherent ability to be tuned by external stimuli will be more versatile. We engineered Escherichia coli cells to behave as an externally tunable band-pass filter for enzyme activity and small molecules. The band's location can be positioned within a range of 4 orders of magnitude simply by the addition of compounds to the growth medium. Inclusion in the genetic network of an enzyme-substrate pair that functions as an attenuator is a generalizable strategy that enables this tunability. The genetic circuit enabled bacteria growth to be patterned in response to chemical gradients in nonintuitive ways and facilitated the isolation of engineered allosteric enzymes. The application of this strategy to other biological systems will increase their utility for biotechnological applications and their usefulness as a tool for gaining insight into nature's underlying design principles.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.