ArticleJournal of bacteriology2009
Replication initiator DnaA of Escherichia coli changes its assembly form on the replication origin during the cell cycle.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- PolyP inhibits CobB deacetylase triggering a regulatory coupling to prevent DNA replication initiation.Nucleic acids research · 2026Article
- The Escherichia coli replication initiator DnaA is titrated on the chromosome.Nature communications · 2025Article
- Relative Distribution of DnaA and DNA inInternational journal of molecular sciences · 2025Article
- IHF and Fis asInternational journal of molecular sciences · 2023Review
- Cell Size Is Coordinated with Cell Cycle by Regulating Initiator Protein DnaA in E. coli.Biophysical journal · 2020Article
- The DnaA Tale.Frontiers in microbiology · 2018Review
- Review
- Rapid turnover of DnaA at replication origin regions contributes to initiation control of DNA replication.PLoS genetics · 2017Article
- Size sensors in bacteria, cell cycle control, and size control.Frontiers in microbiology · 2015Review
- Mutant DnaAs of Escherichia coli that are refractory to negative control.Nucleic acids research · 2013Article
- RecA protein recruits structural maintenance of chromosomes (SMC)-like RecN protein to DNA double-strand breaks.The Journal of biological chemistry · 2013Article
- Helicase loading at chromosomal origins of replication.Cold Spring Harbor perspectives in biology · 2013Review
- Crosstalk between DnaA protein, the initiator of Escherichia coli chromosomal replication, and acidic phospholipids present in bacterial membranes.International journal of molecular sciences · 2013Review
- Chromosome Replication in Escherichia coli: Life on the Scales.Life (Basel, Switzerland) · 2012Article
- Article
- Localization of acidic phospholipid cardiolipin and DnaA in mycobacteria.Tuberculosis (Edinburgh, Scotland) · 2011Article
- Regulation of the replication cycle: conserved and diverse regulatory systems for DnaA and oriC.Nature reviews. Microbiology · 2010Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DnaA is a replication initiator protein that is conserved among bacteria. It plays a central role in the initiation of DNA replication. In order to monitor its behavior in living Escherichia coli cells, a nonessential portion of the protein was replaced by a fluorescent protein. Such a strain grew normally, and flow cytometry data suggested that the chimeric protein has no substantial loss of the initiator activity. The initiator was distributed all over the nucleoid. Furthermore, a majority of the cells exhibited certain distinct foci that emitted bright fluorescence. These foci colocalized with the replication origin (oriC) region and were brightest during the period spanning the initiation event. In cells that had undergone the initiation, the foci were enriched in less intense ones. In addition, a significant portion of the oriC regions at this cell cycle stage had no colocalized DnaA-enhanced yellow fluorescent protein (EYFP) focus point. It was difficult to distinguish the initiator titration locus (datA) from the oriC region. However, involvement of datA in the initiation control was suggested from the observation that, in DeltadatA cells, DnaA-EYFP maximally colocalized with the oriC region earlier in the cell cycle than it did in wild-type cells and oriC concentration was increased.
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