ArticleJournal of bacteriology2009
Multiple replication origins of Halobacterium sp. strain NRC-1: properties of the conserved orc7-dependent oriC1.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- Genomic re-sequencing reveals mutational divergence across genetically engineered strains of model archaea.mSystems · 2025Article
- 'All About' Extremophiles.Faculty reviews · 2023Review
- Recent advances in understanding extremophiles.F1000Research · 2019Review
- Diversity of DNA Replication in the Archaea.Genes · 2017Review
- Activation of a dormant replication origin is essential for Haloferax mediterranei lacking the primary origins.Nature communications · 2015Article
- Article
- Choosing a suitable method for the identification of replication origins in microbial genomes.Frontiers in microbiology · 2015Review
- Association between the dynamics of multiple replication origins and the evolution of multireplicon genome architecture in haloarchaea.Genome biology and evolution · 2014Article
- Recent Advances in the Identification of Replication Origins Based on the Z-curve Method.Current genomics · 2014Article
- The dynamic nature of genomes across the tree of life.Genome biology and evolution · 2014Article
- Multiple replication origins with diverse control mechanisms in Haloarcula hispanica.Nucleic acids research · 2014Article
- DNA replication origins in archaea.Frontiers in microbiology · 2014Review
- The effects of extremes of pH on the growth and transcriptomic profiles of three haloarchaea.F1000Research · 2014Article
- Diversity of the DNA replication system in the Archaea domain.Archaea (Vancouver, B.C.) · 2014Review
- Archaeology of eukaryotic DNA replication.Cold Spring Harbor perspectives in biology · 2013Review
- DoriC 5.0: an updated database of oriC regions in both bacterial and archaeal genomes.Nucleic acids research · 2013Article
- Diversity and evolution of multiple orc/cdc6-adjacent replication origins in haloarchaea.BMC genomics · 2012Article
- Haloquadratum walsbyi: limited diversity in a global pond.PloS one · 2011Article
- Evolutionary rates and gene dispensability associate with replication timing in the archaeon Sulfolobus islandicus.Genome biology and evolution · 2010Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The eukaryote-like DNA replication system of the model haloarchaeon Halobacterium NRC-1 is encoded within a circular chromosome and two large megaplasmids or minichromosomes, pNRC100 and pNRC200. We previously showed by genetic analysis that 2 (orc2 and orc10) of the 10 genes coding for Orc-Cdc6 replication initiator proteins were essential, while a third (orc7), located near a highly conserved autonomously replicating sequence, oriC1, was nonessential for cell viability. Here we used whole-genome marker frequency analysis (MFA) and found multiple peaks, indicative of multiple replication origins. The largest chromosomal peaks were located proximal to orc7 (oriC1) and orc10 (oriC2), and the largest peaks on the extrachromosomal elements were near orc9 (oriP1) in both pNRC100 and -200 and near orc4 (oriP2) in pNRC200. MFA of deletion strains containing different combinations of chromosomal orc genes showed that replication initiation at oriC1 requires orc7 but not orc6 and orc8. The initiation sites at oriC1 were determined by replication initiation point analysis and found to map divergently within and near an AT-rich element flanked by likely Orc binding sites. The oriC1 region, Orc binding sites, and orc7 gene orthologs were conserved in all sequenced haloarchaea. Serial deletion of orc genes resulted in the construction of a minimal strain containing not only orc2 and orc10 but also orc9. Our results suggest that replication in this model system is intriguing and more complex than previously thought. We discuss these results from the perspective of the replication strategy and evolution of haloarchaeal genomes.
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