ArticleJournal of bacteriology2009
Sxy induces a CRP-S regulon in Escherichia coli.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 46 citations in OpenAlex.
- Insight into the environmental cues modulating the expression of bacterial toxin-antitoxin systems.FEMS microbiology reviews · 2025Review
- Type IV minor pilin ComN predicted the USS-receptor in Pasteurellaceae.Frontiers in microbiology · 2025Article
- SwrA-mediated Multimerization of DegU and an Upstream Activation Sequence Enhance Flagellar Gene Expression in Bacillus subtilis.Journal of molecular biology · 2024Article
- Transmissibility and Persistence of the Plasmid-Borne Mobile Colistin Resistance Gene,Antibiotics (Basel, Switzerland) · 2022Article
- Article
- Structure and Assembly of the Enterohemorrhagic Escherichia coli Type 4 Pilus.Structure (London, England : 1993) · 2019Article
- Diversification of the type IV filament superfamily into machines for adhesion, protein secretion, DNA uptake, and motility.PLoS biology · 2019Article
- Functional reconstitution of the type IVa pilus assembly system from enterohaemorrhagic Escherichia coli.Molecular microbiology · 2019Article
- CRP Interacts Specifically With Sxy to Activate Transcription inFrontiers in microbiology · 2019Article
- Horizontal Plasmid Transfer by Transformation inFrontiers in microbiology · 2018Review
- A streptomycin resistance marker inPeerJ · 2018Article
- Identification and Characterization of Domesticated Bacterial Transposases.Genome biology and evolution · 2017Article
- DprA from Neisseria meningitidis: properties and role in natural competence for transformation.Microbiology (Reading, England) · 2017Article
- Silently transformable: the many ways bacteria conceal their built-in capacity of genetic exchange.Current genetics · 2017Review
- Analysis of Streptococcus agalactiae pan-genome for prevalence, diversity and functionality of integrative and conjugative or mobilizable elements integrated in the tRNA(Lys CTT) gene.Molecular genetics and genomics : MGG · 2015Article
- Natural competence and the evolution of DNA uptake specificity.Journal of bacteriology · 2014Review
- Bacterial transformation: distribution, shared mechanisms and divergent control.Nature reviews. Microbiology · 2014Review
- Competence and natural transformation in vibrios.Molecular microbiology · 2013Article
- The availability of purine nucleotides regulates natural competence by controlling translation of the competence activator Sxy.Molecular microbiology · 2013Article
- Multiple Pathways of Genome Plasticity Leading to Development of Antibiotic Resistance.Antibiotics (Basel, Switzerland) · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Escherichia coli is not considered naturally competent, yet it has homologues of the genes that most competent bacteria use for DNA uptake and processing. In Haemophilus influenzae and Vibrio cholerae, these genes are regulated by the Sxy and cyclic AMP receptor (CRP) proteins. We used microarrays to find out whether similar regulation occurs in E. coli. Expression of sxy strongly induced 63 transcriptional units, 34 of which required CRP for transcriptional activation and had promoter sites resembling the Sxy- and CRP-dependent CRP-S motif previously characterized in H. influenzae. As previously reported, sxy expression also induced the sigma-H regulon. Flagellar operons were downregulated by sxy expression, although motility remained unaffected. The CRP-S regulon included all of E. coli's known competence gene homologues, so we investigated Sxy's effect on competence-associated phenotypes. A sxy knockout reduced both "natural" plasmid transformation and competitive fitness in long-term culture. In addition, expression of plasmid-borne sxy led to production of type IV pilin, the main subunit of the DNA uptake machinery of most bacteria. Although H. influenzae Sxy only weakly activated the E. coli Sxy regulon, induction was dramatically improved when it was coexpressed with its cognate CRP, suggesting that intimate interactions between Sxy and CRP are required for transcriptional activation at CRP-S sites.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.