ArticleAmerican journal of respiratory cell and molecular biology2010
Effects of formoterol on contraction and Ca2+ signaling of mouse airway smooth muscle cells.
Article in American journal of respiratory cell and molecular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- Precision cut lung slices: an integrated ex vivo model for studying lung physiology, pharmacology, disease pathogenesis and drug discovery.Respiratory research · 2024Review
- Perspectives on precision cut lung slices-powerful tools for investigation of mechanisms and therapeutic targets in lung diseases.Frontiers in pharmacology · 2023Review
- Soluble Guanylate Cyclase Agonists Induce Bronchodilation in Human Small Airways.American journal of respiratory cell and molecular biology · 2020Article
- Airway smooth muscle dysfunction in Pompe (American journal of physiology. Lung cellular and molecular physiology · 2017Article
- Cryopreserved Human Precision-Cut Lung Slices as a Bioassay for Live Tissue Banking. A Viability Study of Bronchodilation with Bitter-Taste Receptor Agonists.American journal of respiratory cell and molecular biology · 2016Article
- Recruitment of β-arrestin 1 and 2 to the β2-adrenoceptor: analysis of 65 ligands.The Journal of pharmacology and experimental therapeutics · 2015Article
- Bitter tasting compounds dilate airways by inhibiting airway smooth muscle calcium oscillations and calcium sensitivity.British journal of pharmacology · 2014Article
- Mitochondria in lung diseases.Expert review of respiratory medicine · 2013Review
- Hydrogen sulphide inhibits Ca2+ release through InsP3 receptors and relaxes airway smooth muscle.The Journal of physiology · 2013Article
- Ca2+ oscillations, Ca2+ sensitization, and contraction activated by protein kinase C in small airway smooth muscle.The Journal of general physiology · 2013Article
- cAMP regulation of airway smooth muscle function.Pulmonary pharmacology & therapeutics · 2013Review
- Differential effects of formoterol on thrombin- and PDGF-induced proliferation of human pulmonary arterial vascular smooth muscle cells.Respiratory research · 2012Article
- A multi-scale approach to airway hyperresponsiveness: from molecule to organ.Frontiers in physiology · 2012Article
- Exploring lung physiology in health and disease with lung slices.Pulmonary pharmacology & therapeutics · 2011Review
- Bitter treats for better breathing.Nature medicine · 2010Article
- Human airway contraction and formoterol-induced relaxation is determined by Ca2+ oscillations and Ca2+ sensitivity.American journal of respiratory cell and molecular biology · 2010Article
- Nitric oxide induces airway smooth muscle cell relaxation by decreasing the frequency of agonist-induced Ca2+ oscillations.The Journal of general physiology · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Formoterol, a long-acting beta(2)-receptor agonist, is used to relieve bronchial constriction. However, formoterol is often a racemic formulation, and contains both (R,R)- and (S,S)-enantiomers. Because the activity of each isomer is poorly defined, the mechanisms by which formoterol relaxes smooth muscle cells (SMCs) of intrapulmonary airways are not well understood. Consequently, we compared the effects of (S,S)-, (R,R)-, and racemic formoterol, as well as (R)-albuterol, on the contraction and Ca(2+) signaling of airway SMCs in mouse lung slices with phase-contrast and confocal microscopy. Small airways were contracted with methacholine and the associated SMCs displayed sustained Ca(2+) oscillations and an increase in Ca(2+) sensitivity. These contracted airways displayed a substantial, concentration-dependent relaxation in response to (R,R)-formoterol. Racemic formoterol had a similar potency as (R,R)-formoterol for relaxing airways. By contrast, (S,S)-formoterol only induced a small relaxation. In conjunction with relaxation, (R,R)- and racemic formoterol stopped and decreased the methacholine-induced Ca(2+) oscillations and Ca(2+) sensitivity of the SMCs, respectively, whereas (S,S)-formoterol only decreased the Ca(2+) sensitivity. In these studies, (R,R)- and racemic formoterol had a similar, but much greater, potency than (R)-albuterol for relaxing mice airways. This action was quickly initiated at high concentrations by decreasing the frequency of Ca(2+) oscillations, but was more usually mediated at lower concentrations by decreasing the Ca(2+) sensitivity of the SMCs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.