ArticlePlant & cell physiology2009
Proteome analysis of detergent-resistant membranes (DRMs) associated with OsRac1-mediated innate immunity in rice.
Article in Plant & cell physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 79 citations in OpenAlex.
- The function of plant PR1 and other members of the CAP protein superfamily in plant-pathogen interactions.Molecular plant pathology · 2023Review
- Detergent Resistant Membrane Domains in Broccoli Plasma Membrane Associated to the Response to Salinity Stress.International journal of molecular sciences · 2020Article
- Prospects of Gene Knockouts in the Functional Study of MAMP-Triggered Immunity: A Review.International journal of molecular sciences · 2020Review
- Dynamics and Endocytosis of Flot1 inInternational journal of molecular sciences · 2020Article
- Secretion of Phospholipase Dδ Functions as a Regulatory Mechanism in Plant Innate Immunity.The Plant cell · 2019Article
- Plant and animal PR1 family members inhibit programmed cell death and suppress bacterial pathogens in plant tissues.Molecular plant pathology · 2018Article
- Subcellular Proteomics: Application to Elucidation of Flooding-Response Mechanisms in Soybean.Proteomes · 2018Review
- Plasma Membrane Microdomains Are Essential for Rac1-RbohB/H-Mediated Immunity in Rice.The Plant cell · 2016Article
- The synthetic cationic lipid diC14 activates a sector of the Arabidopsis defence network requiring endogenous signalling components.Molecular plant pathology · 2015Article
- Overexpression of BAX INHIBITOR-1 Links Plasma Membrane Microdomain Proteins to Stress.Plant physiology · 2015Article
- Plastid Proteomic Analysis in Tomato Fruit Development.PloS one · 2015Article
- Reversible Dissolution of Microdomains in Detergent-Resistant Membranes at Physiological Temperature.PloS one · 2015Article
- A tribute to Ko Shimamoto (1949-2013).Journal of experimental botany · 2014Article
- Plasma membrane localization is essential for Oryza sativa Pto-interacting protein 1a-mediated negative regulation of immune signaling in rice.Plant physiology · 2014Article
- Dynamic changes in the subcellular distribution of the tobacco ROS-producing enzyme RBOHD in response to the oomycete elicitor cryptogein.Journal of experimental botany · 2014Article
- Interaction of brassinosteroid functions and sucrose transporter SlSUT2 regulate the formation of arbuscular mycorrhiza.Plant signaling & behavior · 2014Article
- Leaf oil body functions as a subcellular factory for the production of a phytoalexin in Arabidopsis.Plant physiology · 2014Article
- Plasma membrane protein trafficking in plant-microbe interactions: a plant cell point of view.Frontiers in plant science · 2014Article
- Quantitative proteomics reveals that plasma membrane microdomains from poplar cell suspension cultures are enriched in markers of signal transduction, molecular transport, and callose biosynthesis.Molecular & cellular proteomics : MCP · 2013Article
- Purification and characterization of novel microtubule-associated proteins from Arabidopsis cell suspension cultures.Plant physiology · 2013Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
OsRac1, a member of the Rac/Rop GTPase family, plays important roles as a molecular switch in rice innate immunity, and the active form of OsRac1 functions in the plasma membrane (PM). To study the precise localization of OsRac1 in the PM and its possible association with other signaling components, we performed proteomic analysis of DRMs (detergent-resistant membranes) isolated from rice suspension-cultured cells transformed with myc-tagged constitutively active (CA) OsRac1. DRMs are regions of the PM that are insoluble after Triton X-100 treatment under cold conditions and are thought to be involved in various signaling processes in animal, yeast and plant cells. We identified 192 proteins in DRMs that included receptor-like kinases (RLKs) such as Xa21, nucleotide-binding leucine-rich repeat (NB-LRR)-type disease resistance proteins, a glycosylphosphatidylinositol (GPI)-anchored protein, syntaxin, NADPH oxidase, a WD-40 repeat family protein and various GTP-binding proteins. Many of these proteins have been previously identified in the DRMs isolated from other plant species, and animal and yeast cells, validating the methods used in our study. To examine the possible association of DRMs and OsRac1-mediated innate immunity, we used rice suspension-cultured cells transformed with myc-tagged wild-type (WT) OsRac1 and found that OsRac1 and RACK1A, an effector of OsRac1, shifted to the DRMs after chitin elicitor treatment. These results suggest that OsRac1-mediated innate immunity is associated with DRMs in the PM.
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