ArticlePlant physiology2009
Apple sucrose transporter SUT1 and sorbitol transporter SOT6 interact with cytochrome b5 to regulate their affinity for substrate sugars.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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47 citing papers in PubMed, 132 citations in OpenAlex.
- Plant SUT Sucrose Transporters: Structure, Evolution and Biological Functions.Current protein & peptide science · 2026Review
- Integrated Transcriptome and Metabolome Analysis of Mature Stage Sand Pear Fruit Response to High-Temperature Stress.Plants (Basel, Switzerland) · 2025Article
- Trichome development of systemic developing leaves is regulated by a nutrient sensor-relay mechanism within mature leaves.Science advances · 2025Article
- Unraveling wheat's response to salt stress during early growth stages through transcriptomic analysis and co-expression network profiling.BMC genomic data · 2024Article
- Phloem unloading in cultivated melon fruits follows an apoplasmic pathway during enlargement and ripening.Horticulture research · 2023Article
- Article
- Long-distance translocation of CLAVATA3/ESR-related 2 peptide and its positive effect on roots sucrose status.Plant physiology · 2022Article
- Characterization and Functional Analysis ofDNA and cell biology · 2022Article
- Integrative Physiological and Transcriptomic Analysis Reveals the Transition Mechanism of Sugar Phloem Unloading Route inInternational journal of molecular sciences · 2022Article
- CytochromeFrontiers in plant science · 2022Review
- Research Progress on Genetic Basis of Fruit Quality Traits in Apple (Frontiers in plant science · 2022Review
- An endoplasmic reticulum-localized cytochromeProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Systematic Genome-Wide Study and Expression Analysis ofInternational journal of molecular sciences · 2021Article
- Hexose transporter CsSWEET7a in cucumber mediates phloem unloading in companion cells for fruit development.Plant physiology · 2021Article
- Shotgun proteomics of peach fruit reveals major metabolic pathways associated to ripening.BMC genomics · 2021Article
- A Cytochrome BFrontiers in plant science · 2021Article
- Nitrogen Levels Regulate Sugar Metabolism and Transport in the Shoot Tips of Crabapple Plants.Frontiers in plant science · 2021Article
- The sucrose transporter MdSUT4.1 participates in the regulation of fruit sugar accumulation in apple.BMC plant biology · 2020Article
- Sugar Signaling During Fruit Ripening.Frontiers in plant science · 2020Review
- Response of Soybean Root to Phosphorus Deficiency under Sucrose Feeding: Insight from Morphological and Metabolome Characterizations.BioMed research international · 2020Article
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sugar transporters are central machineries to mediate cross-membrane transport of sugars into the cells, and sugar availability may serve as a signal to regulate the sugar transporters. However, the mechanisms of sugar transport regulation by signal sugar availability remain unclear in plant and animal cells. Here, we report that a sucrose transporter, MdSUT1, and a sorbitol transporter, MdSOT6, both localized to plasma membrane, were identified from apple (Malus domestica) fruit. Using a combination of the split-ubiquitin yeast two-hybrid, immunocoprecipitation, and bimolecular fluorescence complementation assays, the two distinct sugar transporters were shown to interact physically with an apple endoplasmic reticulum-anchored cytochrome b5 MdCYB5 in vitro and in vivo. In the yeast systems, the two different interaction complexes function to up-regulate the affinity of the sugar transporters, allowing cells to adapt to sugar starvation. An Arabidopsis (Arabidopsis thaliana) homolog of MdCYB5, AtCYB5-A, also interacts with the two sugar transporters and functions similarly. The point mutations leucine-73 --> proline in MdSUT1 and leucine-117 --> proline in MdSOT6, disrupting the bimolecular interactions but without significantly affecting the transporter activities, abolish the stimulating effects of the sugar transporter-cytochrome b5 complex on the affinity of the sugar transporters. However, the yeast (Saccharomyces cerevisiae) cytochrome b5 ScCYB5, an additional interacting partner of the two plant sugar transporters, has no function in the regulation of the sugar transporters, indicating that the observed biological functions in the yeast systems are specific to plant cytochrome b5s. These findings suggest a novel mechanism by which the plant cells tailor sugar uptake to the surrounding sugar availability.
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