ArticleGastroenterology2009
Expression of the chemokine binding protein M3 promotes marked changes in the accumulation of specific leukocytes subsets within the intestine.
Article in Gastroenterology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 31 citations in OpenAlex.
- Diet-Induced Obesity Promotes Liver Metastasis of Pancreatic Ductal Adenocarcinoma via CX3CL1/CX3CR1 Axis.Journal of immunology research · 2022Article
- Disparate Recruitment and Retention of Plasmacytoid Dendritic Cells to The Small Intestinal Mucosa between Young and Aged Mice.Aging and disease · 2021Article
- Deriving Immune Modulating Drugs from Viruses-A New Class of Biologics.Journal of clinical medicine · 2020Review
- The role of chemokine receptor 9/chemokine ligand 25 signaling: From immune cells to cancer cells.Oncology letters · 2018Review
- Article
- The role of apolipoprotein A-IV in regulating glucagon-like peptide-1 secretion.American journal of physiology. Gastrointestinal and liver physiology · 2015Article
- The impact of ATRA on shaping human myeloid cell responses to epithelial cell-derived stimuli and on T-lymphocyte polarization.Mediators of inflammation · 2015Article
- Lloyd F. Mayer, MD: a remembrance.Mucosal immunology · 2014Article
- Lung dendritic cells imprint T cell lung homing and promote lung immunity through the chemokine receptor CCR4.The Journal of experimental medicine · 2013Article
- Mononuclear phagocyte diversity in the intestine.Immunologic research · 2012Review
- The biology of chemokines and their receptors.Immunologic research · 2012Review
- Chemokine 25-induced signaling suppresses colon cancer invasion and metastasis.The Journal of clinical investigation · 2012Article
- Recent advances in small bowel diseases: Part I.World journal of gastroenterology · 2012Review
- Attenuation of TNF-driven murine ileitis by intestinal expression of the viral immunomodulator CrmD.Mucosal immunology · 2010Article
- Modulation of chemokine activity by viruses.Current opinion in immunology · 2010Review
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 4 countries.
Funding
Abstract
BACKGROUND &
aimsChemokines are small proteins that direct leukocyte trafficking under homeostatic and inflammatory conditions. We analyzed the differential expression of chemokines in distinct segments of the intestine and investigated the importance of chemokines for the distribution of leukocytes in the intestine during homeostatic and inflammatory conditions.
methodsWe analyzed messenger RNA for all known chemokines in different segments of the gut by quantitative polymerase chain reaction. To study the effect of multiple-chemokine blockade in the gut, we generated transgenic mice that expressed the chemokine binding protein M3 in the intestine (V-M3 mice). We used flow cytometry to evaluate the changes in the numbers of leukocytes.
resultsWe observed distinct chemokine expression profiles in the 6 segments of the gut. Some chemokines were expressed throughout the intestine (CCL28, CCL6, CXCL16, and CX3CL1), whereas others were expressed preferentially in the small (CCL25 and CCL5) or large intestine (CCL19, CCL21, and CXCL5). Expression of the chemokine blocker M3 in intestinal epithelial cells resulted in reduced numbers of B and T cells in Peyer's patches, reduced numbers of intraepithelial CD8alphabeta(+)/TCRalphabeta(+) and CD8alphaalpha(+)/TCRalphabeta(+) T cells, and reduced numbers of lamina propria CD8(+) T cells. Strikingly, M3 expression markedly reduced the number of eosinophils and macrophages in the small and large intestines. Dextran sulfate sodium treatment of control mice led to marked changes in the expression of chemokines and in the number of myeloid cells in the colon. These cellular changes were significantly attenuated in the presence of M3.
conclusionsOur study reveals a complex pattern of chemokine expression in the intestine and indicates that chemokines are critical for leukocyte accumulation in the intestine during homeostasis and inflammation.
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