ArticleFree radical biology & medicine2009
Role of glycocalyx in flow-induced production of nitric oxide and reactive oxygen species.
Article in Free radical biology & medicine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
51 citing papers in PubMed, 99 citations in OpenAlex.
- Activation of the C3a-C3aReceptor-axis is associated with endothelial dysfunction and glycocalyx damage in ST-elevation myocardial infarction.Basic research in cardiology · 2026Article
- Endothelial CD44 cleavage by ADAM17 impairs shear stress mechanotransduction.Function (Oxford, England) · 2026Article
- Review
- Multimodal Profiling Reveals Distinct Endothelial Activation Pathways Regulated by Flow and Heparan Sulfate.Cellular and molecular bioengineering · 2026Article
- Heparan Sulfates Regulate Vascular Reactivity in Syndecan 1 Knockout Mice.International journal of molecular sciences · 2026Article
- Redox Regulation of Microvascular Physiology and Pathophysiology: Insights into Therapeutic Strategies and Limitations.Antioxidants & redox signaling · 2025Review
- CD44 signaling in skin wound healing and regeneration.Journal of translational medicine · 2025Review
- A Theoretical Analysis of the Effects That the Glycocalyx and the Internal Elastic Lamina Have on Nitric Oxide Concentration Gradients in the Arterial Wall.Antioxidants (Basel, Switzerland) · 2025Article
- Acute neuroendocrine challenge elicits enhanced cortisol response and parallel transcriptomic changes in patients with migraine.Pain reports · 2025Article
- Integrating molecular and cellular components of endothelial shear stress mechanotransduction.American journal of physiology. Heart and circulatory physiology · 2024Review
- Mechanosensing by Vascular Endothelium.Annual review of physiology · 2024Review
- Effect of acute heparin administration on glycocalyx thickness and endothelial function in healthy younger adults.Journal of applied physiology (Bethesda, Md. : 1985) · 2024Article
- Unraveling neurovascular mysteries: the role of endothelial glycocalyx dysfunction in Alzheimer's disease pathogenesis.Frontiers in physiology · 2024Review
- Neuraminidase inhibition improves endothelial function in diabetic mice.American journal of physiology. Heart and circulatory physiology · 2023Article
- Coronary "Microvascular Dysfunction": Evolving Understanding of Pathophysiology, Clinical Implications, and Potential Therapeutics.International journal of molecular sciences · 2023Review
- Mechanisms of endothelial flow sensing.Nature cardiovascular research · 2023Review
- Vascular mechanotransduction.Physiological reviews · 2023Review
- Review
- Two Methods of AuNPs Synthesis Induce Differential Vascular Effects. The Role of the Endothelial Glycocalyx.Frontiers in medicine · 2022Article
- Glycocalyx sialic acids regulate Nrf2-mediated signaling by fluid shear stress in human endothelial cells.Redox biology · 2021Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Although the glycocalyx has been implicated in wall shear stress (WSS) mechanotransduction, the role of glycocalyx components in nitric oxide (NO) and reactive oxygen species (ROS) production remains unclear. Here, we tested the hypothesis that glycocalyx is implicated in both endothelial NO and O(2)(-) production. Specifically, we evaluated the role of hyaluronic acid (HA), heparan sulfate (HS), and sialic acid (SA) in NO and O(2)(-) mechanotransduction. Twenty-seven ex vivo porcine superficial femoral arteries were incubated with heparinase III, hyaluronidase, or neuraminidase, to remove HS, HA, or SA, respectively, from glycocalyx. The arteries were then subjected to steady-state flow and the effluent solution was measured for nitrites and the vessel diameter was tracked to quantify the degree of vasodilation. Our results show that removal of HA decreased both nitrites and vasodilation, and tempol treatment had no reversing effect. Degradation of HS proteoglycans decreased NO bioavailability through an increase in O(2)(-) production as indicated by fluorescent signals of dihydroethidium (DHE) and its area fraction (209+/-24% increase) and also removed extracellular O(2)(-) dismutase (ecSOD) (67+/-9% decrease). The removal of SA also increased O(2)(-) production as indicated by DHE fluorescent signals (86+/-17% increase) and the addition of tempol, a mimic O(2)(-) scavenger, restored both NO availability and vasodilation in both heparinase- and neuraminidase-treated vessels. This implies that HS and SA are not directly involved in WSS-mediated NO production. This study implicates HA in WSS-mediated NO mechanotransduction and underscores the role of HS and SA in ROS regulation in vessel walls in response to WSS stimulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.