ArticleJournal of hematology & oncology2009
Identification of circulating tumour cells in early stage breast cancer patients using multi marker immunobead RT-PCR.
Article in Journal of hematology & oncology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 34 citations in OpenAlex.
- Perioperative circulating tumor cell detection: Current perspectives.Cancer biology & therapy · 2016Review
- Minimal residual disease in breast cancer: an overview of circulating and disseminated tumour cells.Clinical & experimental metastasis · 2016Review
- An optimised direct lysis method for gene expression studies on low cell numbers.Scientific reports · 2015Article
- Mesenchymal phenotype of CTC-enriched blood fraction and lymph node metastasis formation potential.PloS one · 2014Article
- Evaluation of a multi-marker immunomagnetic enrichment assay for the quantification of circulating melanoma cells.Journal of translational medicine · 2012Article
- Methylation of cancer related genes in tumor and peripheral blood DNA from the same breast cancer patient as two independent events.Diagnostic pathology · 2011Article
- Circulating tumor cells in breast cancer patients: an evolving role in patient prognosis and disease progression.Pathology research international · 2011Article
- Constitutional methylation of the BRCA1 promoter is specifically associated with BRCA1 mutation-associated pathology in early-onset breast cancer.Cancer prevention research (Philadelphia, Pa.) · 2011Article
- Long term survival following the detection of circulating tumour cells in head and neck squamous cell carcinoma.BMC cancer · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionThe ability to screen blood of early stage operable breast cancer patients for circulating tumour cells is of potential importance for identifying patients at risk of developing distant relapse. We present the results of a study of the efficacy of the immunobead RT-PCR method in identifying patients with circulating tumour cells.
resultsImmunomagnetic enrichment of circulating tumour cells followed by RT-PCR (immunobead RT-PCR) with a panel of five epithelial specific markers (ELF3, EPHB4, EGFR, MGB1 and TACSTD1) was used to screen for circulating tumour cells in the peripheral blood of 56 breast cancer patients. Twenty patients were positive for two or more RT-PCR markers, including seven patients who were node negative by conventional techniques. Significant increases in the frequency of marker positivity was seen in lymph node positive patients, in patients with high grade tumours and in patients with lymphovascular invasion. A strong trend towards improved disease free survival was seen for marker negative patients although it did not reach significance (p = 0.08).
conclusionMulti-marker immunobead RT-PCR analysis of peripheral blood is a robust assay that is capable of detecting circulating tumour cells in early stage breast cancer patients.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.