ArticleHistochemistry and cell biology2009
Characterisation and expression of SPLUNC2, the human orthologue of rodent parotid secretory protein.
Article in Histochemistry and cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 40 citations in OpenAlex.
- Peroxisomes Are Highly Abundant and Heterogeneous in Human Parotid Glands.International journal of molecular sciences · 2023Article
- Longitudinal efficacy and toxicity of SARS-CoV-2 vaccination in cancer patients treated with immunotherapy.Cell death & disease · 2023Article
- Functional Specialization of Human Salivary Glands and Origins of Proteins Intrinsic to Human Saliva.Cell reports · 2020Article
- Profiling Autoantibodies against Salivary Proteins in Sicca Conditions.Journal of dental research · 2019Article
- Glycan recognition at the saliva - oral microbiome interface.Cellular immunology · 2018Review
- Changes in salivary analytes in canine parvovirus: A high-resolution quantitative proteomic study.Comparative immunology, microbiology and infectious diseases · 2018Article
- Salivary proteomics of healthy dogs: An in depth catalog.PloS one · 2018Article
- Alterations in the Salivary Proteome and N-Glycome of Sjögren's Syndrome Patients.Journal of proteome research · 2017Article
- Allergen ligands in the initiation of allergic sensitization.Current allergy and asthma reports · 2014Article
- Antimicrobial peptides in periodontal innate defense.Frontiers of oral biology · 2012Review
- Human parotid secretory protein is a lipopolysaccharide-binding protein: identification of an anti-inflammatory peptide domain.Molecular and cellular biochemistry · 2012Article
- Dual host-defence functions of SPLUNC2/PSP and synthetic peptides derived from the protein.Biochemical Society transactions · 2011Review
- Identification and characterisation of the BPI/LBP/PLUNC-like gene repertoire in chickens reveals the absence of a LBP gene.Developmental and comparative immunology · 2011Article
- Human LPLUNC1 is a secreted product of goblet cells and minor glands of the respiratory and upper aerodigestive tracts.Histochemistry and cell biology · 2010Article
- Inducible innate resistance of lung epithelium to infection.Annual review of physiology · 2010Review
- Extending the knowledge in histochemistry and cell biology.Histochemistry and cell biology · 2010Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
We recently described the Palate Lung Nasal Clone (PLUNC) family of proteins as an extended group of proteins expressed in the upper airways, nose and mouth. Little is known about these proteins, but they are secreted into the airway and nasal lining fluids and saliva where, due to their structural similarity with lipopolysaccharide-binding protein and bactericidal/permeability-increasing protein, they may play a role in the innate immune defence. We now describe the generation and characterisation of novel affinity-purified antibodies to SPLUNC2, and use them to determine the expression of this, the major salivary gland PLUNC. Western blotting showed that the antibodies identified a number of distinct protein bands in saliva, whilst immunohistochemical analysis demonstrated protein expression in serous cells of the major salivary glands and in the ductal lumens as well as in cells of minor mucosal glands. Antibodies directed against distinct epitopes of the protein yielded different staining patterns in both minor and major salivary glands. Using RT-PCR of tissues from the oral cavity, coupled with EST analysis, we showed that the gene undergoes alternative splicing using two 5' non-coding exons, suggesting that the gene is regulated by alternative promoters. Comprehensive RACE analysis using salivary gland RNA as template failed to identify any additional exons. Analysis of saliva showed that SPLUNC2 is subject to N-glycosylation. Thus, our study shows that multiple SPLUNC2 isoforms are found in the oral cavity and suggest that these proteins may be differentially regulated in distinct tissues where they may function in the innate immune response.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.