ArticleProceedings of the National Academy of Sciences of the United States of America2009
Olfactory neural circuitry for attraction to amino acids revealed by transposon-mediated gene trap approach in zebrafish.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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66 citing papers in PubMed, 159 citations in OpenAlex.
- Dynamic interactions between epithelial skin cells and a sensory cavity sculpt the growing olfactory orifice.Nature communications · 2026Article
- Gut distension evokes rapid neural dynamics in vagal and hindbrain populations of larval zebrafish.iScience · 2026Article
- Hagfish olfactory repertoire illuminates lineage-specific diversification of olfaction in basal vertebrates.iScience · 2025Article
- Structural Regeneration and Functional Recovery of the Olfactory System of Adult Zebrafish Following Brain Injury.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Olfactory Imprinting to Amino Acids is Unpredictable but Specific.Journal of chemical ecology · 2025Article
- Behavioral, Endocrine, and Neuronal Responses to Odors in Lampreys.Animals : an open access journal from MDPI · 2025Review
- Olfactory Dysfunction in a Novel Model of Prodromal Parkinson's Disease in Adult Zebrafish.International journal of molecular sciences · 2025Article
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- Single-Cell RNA-Sequencing of Zebrafish Olfactory Epithelium Identifies Odor-Responsive Candidate Olfactory Receptors.Genes to cells : devoted to molecular & cellular mechanisms · 2025Article
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- Calcium imaging of adult olfactory epithelium reveals amines as important odor class in fish.Cell and tissue research · 2024Article
- The forkhead transcription factor Foxj1 controls vertebrate olfactory cilia biogenesis and sensory neuron differentiation.PLoS biology · 2024Article
- Warming affects routine swimming activity and novel odour response in larval zebrafish.Scientific reports · 2023Article
- Pheromone Perception in Fish: Mechanisms and Modulation by Internal Status.Integrative and comparative biology · 2023Review
- Remarkable diversity of vomeronasal type 2 receptor (OlfC) genes of basal ray-finned fish and its evolutionary trajectory in jawed vertebrates.Scientific reports · 2022Article
- A scalable assay for chemical preference of small freshwater fish.Frontiers in behavioral neuroscience · 2022Article
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- Deletion of lrrk2 causes early developmental abnormalities and age-dependent increase of monoamine catabolism in the zebrafish brain.PLoS genetics · 2021Article
- Larval Zebrafish Use Olfactory Detection of Sodium and Chloride to Avoid Salt Water.Current biology : CB · 2021Article
- Odor hedonics coding in the vertebrate olfactory bulb.Cell and tissue research · 2021Review
6 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In fish, amino acids are food-related important olfactory cues to elicit an attractive response. However, the neural circuit underlying this olfactory behavior is not fully elucidated. In the present study, we applied the Tol2 transposon-mediated gene trap method to dissect the zebrafish olfactory system genetically. Four zebrafish lines (SAGFF27A, SAGFF91B, SAGFF179A, and SAGFF228C) were established in which the modified transcription activator Gal4FF was expressed in distinct subsets of olfactory sensory neurons (OSNs). The OSNs in individual lines projected axons to partially overlapping but mostly different glomeruli in the olfactory bulb (OB). In SAGFF27A, Gal4FF was expressed predominantly in microvillous OSNs innervating the lateral glomerular cluster that corresponded to the amino acid-responsive region in the OB. To clarify the olfactory neural pathway mediating the feeding behavior, we genetically expressed tetanus neurotoxin in the Gal4FF lines to block synaptic transmission in distinct populations of glomeruli and examined their behavioral response to amino acids. The attractive response to amino acids was abolished only in SAGFF27A fish carrying the tetanus neurotoxin transgene. These findings clearly demonstrate the functional significance of the microvillous OSNs innervating the lateral glomerular cluster in the amino acid-mediated feeding behavior of zebrafish. Thus, the integrated approach combining genetic, neuroanatomical, and behavioral methods enables us to elucidate the neural circuit mechanism underlying various olfactory behaviors in adult zebrafish.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.