ArticleJournal of translational medicine2009
Isolation and culture of fibroblasts from endoscopic duodenal biopsies of celiac patients.
Article in Journal of translational medicine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 12 citations in OpenAlex.
- Prognostic significance of fibroblast senescence and senescence-associated secretory phenotype factor expression in the tumor microenvironment of pancreatic ductal adenocarcinoma.Oncology reports · 2026Article
- ROS release by PPARβ/δ-null fibroblasts reduces tumor load through epithelial antioxidant response.Oncogene · 2018Article
- Isolation of CD 90+ Fibroblast/Myofibroblasts from Human Frozen Gastrointestinal Specimens.Journal of visualized experiments : JoVE · 2016Article
- Non-celiac gluten sensitivity: Time for sifting the grain.World journal of gastroenterology · 2015Review
- Restricted VH/VL usage and limited mutations in gluten-specific IgA of coeliac disease lesion plasma cells.Nature communications · 2014Article
- Extracellular matrix proteins and displacement of cultured fibroblasts from duodenal biopsies in celiac patients and controls.Journal of translational medicine · 2013Article
- Immunological comorbity in coeliac disease: associations, risk factors and clinical implications.Journal of clinical immunology · 2012Article
- Congestive heart failure effects on atrial fibroblast phenotype: differences between freshly-isolated and cultured cells.PloS one · 2012Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFibroblasts are actually considered pivotal in inflammation and tissue remodelling process and for these reasons they are involved in the pathogenesis of autoimmune disorders such as celiac disease. Investigations to define the role of fibroblasts in celiac diseases are obstructed by the absence of specific models. Our objective is to isolate and culture primary fibroblasts from endoscopic duodenal biopsies of celiac and non-celiac subjects, to analyze their growth patterns and the morphometric characteristics.
methods60 duodenal bioptic specimens from 20 celiac patients and 114 from 38 non-celiac subjects were mechanically chopped and enzymatically digested in order to obtain primary cell cultures. Growth patterns, karyotype (Q-banding analysis), expression of typing proteins (fibroblast surface protein and cytokeratin 20) and morphometric parameters (diameters and their ratio, perimeter, area and perimeter/area ratio at computerised image analysis) were investigated on cultured cells.
resultsPrimary cells were successfully cultured in 78% of the collected duodenal biopsies. Cultured cells, expressing the fibroblast surface protein, were negative for cytokeratine 20 and maintained a normal kariotype. Cells grew slowly without differences between the celiac and the non celiac group. Morphometric analysis of celiac fibroblasts revealed significantly increased dimensions, with a preserved diameters ratio, and a reduced perimeter/area ratio.
conclusionFor the first time this study demonstrates the feasibility of culturing primary fibroblast cell from endoscopic duodenal biopsies in celiac and non-celiac subjects, opening a new window of opportunity in studies intended to establish the role of fibroblasts as a possible partaker in the pathogenesis of the celiac mucosal damage.
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